Assembly principles of the CO2 -fixing liquid-liquid phase separated organelle, the Pyrenoid

类核蛋白是存在于各种真核藻类叶绿体中的液-液相分离的细胞器，并且负责约30%的全球碳固定。它们是CO2固定酶Rubisco在类囊体膜周围相分离的组装体。该项目将使用尖端的方法对蛋白核的结构和功能进行前所未有的深入研究。参与蛋白核组装的关键Rubisco结合蛋白将在不同的藻类中进行生物信息学鉴定，然后使用尖端的藻类分子生物学和生物化学进行功能表征。它们与Rubisco结合时的三维结构将用冷冻电子显微镜测定。生成的数据将有助于指导将蛋白核改造成农作物，这有可能提高光合作用和产量高达60%。

Pyrenoids are liquid-liquid phase separated organelles found in the chloroplasts of diverse eukaryotic algae and are responsible for ~30%of global carbon fixation. They are assemblies of the CO2 fixing enzyme Rubisco phase-separated around thylakoid membranes. Thisproject will use cutting-edge methods to give unprecedented insights into the structure and function of pyrenoids. Key Rubisco bindingproteins involved in pyrenoid assembly will be identified bioinformatically across diverse algae and then functionallycharacterized using cutting-edge algal molecular biology and biochemistry. Their 3D structure when bound to Rubisco will bedetermined using cryo electron microscopy. Generated data will help guide the engineering of a pyrenoid into crop plants which has thepotential to boost photosynthesis and yields by up to 60%.