Genes & Gestational DM: Case Control Association Study

描述（由申请人提供）：妊娠糖尿病（GDM）是指在怀孕期间首次诊断出的糖尿病；它发生在所有怀孕的7%。许多患有GDM的女性在晚年被诊断为2型糖尿病（T2DM）。某些基因与2型糖尿病相关，但尚未有研究确定这些基因是否与GDM相关。这项病例对照关联研究，根据年龄、种族和体重指数对女性进行匹配，将在患有GDM的女性和没有GDM的女性中检测与2型糖尿病相关的基因。这些基因包括PPAR γ、B3-AR、GRL和CAPN10。从血液样本中提取DNA。聚合酶链反应将用于扩增4个基因，片段大小分析将确定每个样本中存在哪些基因变体。卡方分析将比较GDM女性与非GDM女性个体基因变异的频率。这项研究将有助于了解GDM和T2DM的遗传学。鉴定与糖尿病相关的基因可能有助于干预预防发病或改善与糖尿病相关的并发症。

DESCRIPTION (provided by applicant): Gestational Diabetes (GDM) is diabetes that is first diagnosed during pregnancy; it occurs in 7% of all pregnancies. Many women with GDM are diagnosed with Type 2 Diabetes (T2DM) later in life. Certain genes are associated with T2DM but studies have not been done to determine whether these genes are associated with GDM. This case-control-association-study, matching women for age, ethnicity and body mass index will examine genes associated withT2DM in women with GDM as compared to those without. These genes include the PPAR gamma, B3-AR, GRL and CAPN10. From blood samples, DNA will be extracted. Polymerase Chain Reaction will be used to amplify the 4 genes and fragment size analysis will determine which of the gene variants are present in each sample. Chi square analysis will compare the frequency of the individual gene variants in women with GDM vs. those without. This study will contribute to an understanding of the genetics of GDM and T2DM. Identification of genes associated with diabetes may facilitate interventions to prevent the onset or ameliorate the complications associated with diabetes.