Regulation of Polarised Trafficking Machinery in Epithelial to Mesenchymal Transition.

在上皮向间充质转化(EMT)的过程中，上皮细胞失去了其顶底极性，并获得了侵袭性。目前，尚不清楚调解两极分化人口贩运的贩运机制的监管是如何改变的。包括在贩运机制中的是排囊复合体。肿瘤的关键标志之一是侵袭和转移的激活，这与患者预后不良直接相关。细胞接受EMT的能力是转移的核心。因此，必须更好地了解促进这一步骤的两极分化贩运的变化。为了解决这些问题，在EMT过程中，将使用定量质谱学来鉴定胞囊相互作用蛋白。这项研究将提供对现代医疗保健中的主要挑战之一的洞察。此外，还将使用细胞生物学和重组蛋白生物化学方法进一步描述基底膜上的胞囊动力学和胞囊相互作用的作用。

During epithelial to mesenchymal transition (EMT), epithelial cells lose their apical-basal polarity and gain invasive properties. Currently, it is unknown how the regulation of trafficking machinery which mediates polarised trafficking is altered. Included in the trafficking machinery is the exocyst complex. One of the key hallmarks of cancer is the activation of invasion and metastasis, this directly correlates with a poor prognosis in patients. The ability of cells to undergo EMT is central to metastasis. It is therefore essential to better understand changes to polarised trafficking which facilitate this step. To address these questions, quantitative mass spectrometry will be used to identify exocyst interacting proteins during EMT. This research willprovide an insight into one of the major challenges in modern healthcare. Moreover, cell biology and recombinant protein biochemistry methods will be used to further characterise exocyst dynamics at the basal membrane and the role of exocyst interactors