ANIMAL / PHYSIOLOGY CORE

动物/生理学核心(9002)为所有项目提供支持。每个项目使用由Core获得、安置和维护的Fischer 344只大鼠。该中心为老年心脏研究中使用的无特定病原体的大鼠提供了一个集中的最先进的屏障设施。该中心提供符合老年学研究所需的实验动物健康标准的动物护理，包括无病原体环境、日常一般健康状况评估、体格检查以及体重和肾功能测量。核心位于路易斯托克斯VAMC动物资源设施内的专用空间内。 Core提供的心脏模型为所有项目提供心脏组织。心脏组织是从安乐死后立即切除的心脏中获得的，用于研究处于基线状态的心脏；以及从隔离的缓冲灌流心脏实验中获得。心脏灌流方案包括时间控制灌流、单纯缺血和长达120分钟的再灌注期。采用了两种离体灌流心脏模型，即朗宁多夫模型和工作心脏模型。修订后的申请书中提出的研究使用了目前的两种模式以及新的方法。核心将灌流分离的心脏，以准备组织，以分离心肌细胞到0003。 核心人员将与0003合作，将体内心脏基因转移的模型带到核心。该核心将促进项目间合作研究：阿米塔尔干预和谷氧还蛋白干预。这些研究最大限度地实现了项目之间的组织共享。此外，Core协调四个项目之间的相互作用，以共享未处理的大鼠在缺血前、缺血后和随后的基线状态下的组织 缺血和再灌流。 心脏组织被提供给CORE-9004，用于分离心肌线粒体并用于光学和电子显微镜分析。额外的心脏组织被直接提供给生化和分子研究项目。多余的心脏组织样本被编目并储存起来，以备将来使用。实验被输入一个公共数据库，所有项目都可以通过网络获得。

The Animal/Physiology Core (9002) provides support to all projects. Each project uses Fischer 344 rats that are acquired, housed, and maintained by the Core. The Core provides a centralized state of the art barrier facility for the housing of specific pathogen free rats used in the studies of the aged heart. The Core provides animal care that complies with standards of health for experimental animals required for gerontological studies, including a pathogen free environment, assessment of general health status on a daily basis, physical examination and measurements of body weight and renal function. The Core is located within dedicated space within the Animal Resource Facility of the Louis Stokes VAMC. The cardiac models supplied by the Core provide cardiac tissue to all of the projects. Cardiac tissue is obtained from hearts excised immediately following euthanasia for the study of the heart in the baseline state; and from isolated, buffer perfused heart experiments. Heart perfusion protocols include time control perfusions, ischemia alone, and reperfusion periods of up to 120 minutes. Two isolated perfused heart models have been employed, the Langendorff model and the working heart model. The studies proposed in the revised application use the two current models as well as new approaches. The Core will perfuse isolated hearts to prepare tissue for the isolation of cardiac myocytes by 0003. Core personnel will collaborate with 0003 to bring the model of in vivo cardiac gene transfer to the Core. The Core will facilitate the Inter-project Collaborative Studies: Amytal-Intervention and the Glutaredoxin-Intervention. These studies maximize sharing of tissues among projects. In addition, the Core co-ordinates interactions among the four projects to share tissues from untreated rats in the baseline state prior to ischemia, following ischemia, and following ischemia and reperfusion. Cardiac tissue is provided to core-9004 for the isolation of cardiac mitochondria and for analysis by light and electron microscopy. Additional cardiac tissue is provided directly to the projects for biochemical and molecular studies. Excess cardiac tissue samples are catalogued and stored for future use. Experiments are entered into a common database available via network to all projects.