Biometry and Microarray Core

计算和功能基因组学核心将为所有项目服务，协助研究人员进行研究设计、数据处理和管理，以及微阵列实验的所有技术方面。主要活动将包括设计和处理cDNA、寡核苷酸、蛋白质和抗体阵列实验的结果，通过这些实验分析暴露在低氧或高二氧化碳环境中的小鼠样本中基因表达、蛋白质丰度和蛋白质-蛋白质相互作用的变化。这一核心设施的具体目标如下： 1)在基因组研究的所有方面提供咨询、培训以及技术和生物信息援助，从实验设计和与阵列的RNA和蛋白质杂交到质量控制、数据处理和管理。 2)协助这些项目将其微阵列数据存储在NCBI的GEO数据库中，组织和维护野生型和转基因小鼠低氧影响的转录和蛋白质组数据库，以探索适应或损伤的具体机制，并在同行评议期刊上发表后将这些数据库纳入计划项目的网站。 3)在使用先进的统计方法和数学建模方面提供咨询和培训，并协助调查人员进行研究设计、数据处理和管理。

The Computational and Functional Genomics Core will serve all projects, assisting the investigators in research design and data processing and management, as well as in all technical aspects of microarray experiments. A major activity will consist of designing and processing the results of cDNA, oligonucleotide, protein and antibody array experiments by which to analyze alteration of gene expression, protein abundance and protein-protein interaction within specimens of mice exposed to hypoxia or hypercapnia. The Specific Aims of this Core facility are as follows: 1) To provide consultation, training and technical and bioinformatic assistance in all aspects of genomic studies, from experimental design and RNA and protein hybridization with the arrays to quality control, data processing and management. 2) To assist the projects to deposit their microarray data in the GEO database of NCBI, to organize and maintain transcriptomic and proteomic dbases of hypoxia effects in wildtype and in genetically modified mice to probe specific mechanisms for adaptation or injury and include these databases into the website of the Program Project after publication in peer-reviewed journals. 3) To provide consultation and training in the use of advanced statistical methods and mathematical modeling and to assist investigators in research design, data processing and management.