Quantitative Assessment of Angiogenesis By Perfusion MRI

描述（由申请人提供）： 广泛的长期目标：开发使用动态对比增强灌注MRI（pMRI）评估脑肿瘤血管生成的方法。健康相关性：该项目将应用pMRI评估接受抗血管生成和其他化疗治疗的脑肿瘤。然而，开发的方法也将是有用的，在评估肿瘤性病变以外的脑和非肿瘤性病变innerit. Specific目的：1）调查的假设，pMRI测量脑血容量（CBV）和血管通透性与血管和血脑屏障（BBB）的破坏分别与组织学评估。2)确定pMRI是否可用于脑肿瘤分级。3)探讨pMRI可用于监测化疗疗效的假设。研究设计：1）将在7 T动物成像仪上用pMRI对鼠胶质瘤进行成像。CBV和血管通透性的MRI测量将分别与血管密度和伊文思蓝外渗的组织学评估相关。2)将在组织学证实的不同级别脑肿瘤患者中测量CBV和血管通透性，以确定这些测量值是否可区分它们。3)将对参加新化疗药物试验的胶质瘤患者进行系列pMRI测量：沙利度胺联合卡铂和烷化剂替莫达和CPT-11。MRI测量将与肿瘤大小和临床结果相关，以确定pMRI血管生成指征是否预测肿瘤复发。

DESCRIPTION (provided by applicant): Broad long-term objectives: To develop methods of assessing angiogenesis in brain tumors using dynamic, contrast enhanced perfusion MRI (pMRI). Health-relatedness: The project will apply pMRI to the evaluation of brain tumors undergoing antioangiogenic and other chemotherapeutic treatments. However, the developed methods will also be useful in evaluating neoplastic lesions outside the brain and non-neoplastic lesions within it. Specific aims: 1) To investigate the hypothesis that pMRI measurements of cerebral blood volume (CBV) and vascular permeability correlate with histological assessments of vascularity and blood-brain barrier (BBB) breakdown respectively. 2) To determine whether pMRI can be used to grade brain tumors. 3) To investigate the hypothesis that pMRI can be used to monitor the efficacy of chemotherapeutic treatments. Research design: 1) Murine gliomas will be imaged with pMRI on a 7T animal imager. MRI measurements of CBV and vascular permeability will be correlated with histological assessments of vascular density and Evan's blue extravasation respectively. 2) CBV and vascular permeability will be measured in patients with histologically confirmed brain tumors of different grades to determine whether these measurements differentiate between them. 3) Serial pMRI measurements will be made on glioma patients enrolled in trials of new chemotherapeutic agents: thalidomide combined with carboplatin and the alkylating agents temodar and CPT-11. MRI measurements will be correlated with tumor size and clinical outcome to determine whether pMRI indications of angiogenesis predict tumor recurrence.