Crops & Soil - Crosstalk between the circadian clock and innate immune system in plants

生物钟在人类健康的许多方面的参与是公认的，但植物免疫中的生物钟输入还没有得到很好的理解。由于植物病害造成的农业损失很大，基础植物健康研究有助于应对为日益增长的人口提供食物的人类挑战。植物免疫途径包括关键的翻译后事件，特别是激酶信号级联和与氧化/还原爆发相关的基于氧化还原的修饰，该博士项目使用植物拟南芥和细菌病原体假单胞菌之间的相互作用来测试免疫途径和生物钟之间的特定串扰点。研究生物钟之间的串扰。高度复杂的时钟和免疫信号网络构成了一个具有挑战性的项目，该项目将在两个领域的先进技术中开发候选人，包括时钟基因表达的时间序列荧光素酶成像，详细的qRT-PCR实验和免疫测定。此外，该项目将涉及大量的蛋白质生物化学，包括先进的免疫沉淀技术。候选人将拥有自己的项目，表现出主动性，并在货车Ooijen和Spoel实验室（分别为时钟研究和免疫研究）之间进行有效沟通。

Involvement of the circadian clock in many aspects of human health is well recognised, but circadian input in plant immunity is not as well understood. As agricultural losses from plant disease are significant, fundamental plant health research contributes to the human challenges of providing the increasing population with food. Plant immune pathways include key post-translational events, notably kinase signalling cascades and redox-based modifications associated with oxidative/reductive bursts, which could constitute entry points for clock regulation of defence signalling.This PhD project uses the interaction between the plant Arabidopsis and the bacterial pathogen Pseudomonas to test the specific points of cross talk between immune pathways and the circadian clock.Studying crosstalk between the highly complex networks of clock and immune signalling constitutes a challenging project that will develop the candidate in advanced techniques from both fields, including time series luciferase imaging of clock gene expression, detailed qRT-PCR experiments, and immune assays. Additionally, the project will involve substantial amounts of protein biochemistry, including advanced immunoprecipitation techniques. The candidate will take ownership or their projects, show initiative, and communicate effectively between the van Ooijen and Spoel laboratories (clock research and immune research, respectively).