Space WOrMS whole organism metabolomics

对人体的物理和化学特性进行有效建模是一项挑战。这是因为人类是大型复杂的动物，尚未完全了解，并且可能在科学和社会经济方面都具有挑战性。因此，国际上有一股强烈的动力，要在研究中取代、减少和改进动物的使用，以便保护这种宝贵的资源。秀丽线虫是一种自由生活的蠕虫，是地球上了解最全面的动物。这是因为它的尺寸小（<1 mm），世代时间短（<3天），光学透明度，遗传变异的可用性和内政部动物法规的排除。研究这种生物的研究人员因在遗传学、绿色荧光蛋白标记和RNA干扰方面的进展而获得诺贝尔奖。迄今为止，已获得了C. elegans已经被绘制出来了。然而，目前还没有现成的代谢信息的C。该项目将利用诺丁汉大学药学院和诺丁汉大学最先进的设施，填补生物代谢组学的重要知识空白。液相色谱法将用于解读线虫的代谢组，以确定代谢组相似性和差异的全球趋势。将使用表面敏感质谱法在代谢组中空间协调代谢变化。原子力显微镜将用于了解物理特性。将开发新的显微镜和生物信息学工具，以简化数据分析和组织。作为该项目的一部分，开发的科学第一项将用于物理学、生物学和化学前沿的令人兴奋的研究。这将是一个宝贵的资源，研究人员，以提高他们的理解，整个生物体的生理状态和那些进一步的知识，C。作为复杂哺乳动物生物化学的模型。这将包括优化疾病预测，诊断和干预。该项目还将为博士候选人提供多样化的培训机会。在经验丰富的监督团队的支持下。进行的研究将为建立新的和改进的药物输送模型和分析铺平道路，以增加对整个生物体代谢组学的理解。这也将产生高影响力的出版物和机会，在国际会议上传播研究。

Effective modelling of the physical and chemical properties in humans is challenging. This is because humans are large complex animals, which are not completely understood and can be both scientifically and socio-economically challenging to characterise. Therefore, there is a strong international drive to replace, reduce and refine the use of animals in research so that this precious resource is reserved.C. elegans, a free-living worm, is the most completely understood animal on the planet. This is due to its small size (<1 mm), short generation time (<3 days), optical transparency, availability of genetic variants and exclusion from Home Office animal regulations. Researchers working with this organism have received Nobel prizes for advances in genetics, green fluorescent protein labelling and RNA interference. To date, the complete genome, proteome and connectome for C. elegans have been mapped. However, currently there is no readily available metabolic information on C. elegans.This project will fill the important knowledge gaps in organismal metabolomics in by harnessing the state-of-the-art facilities at the School of Pharmacy and University of Nottingham. Liquid chromatography will be used to decipher metabolomes for nematode to identify global trends in metabolome similarities and differences. Surface sensitive mass spectrometry will be used to spatially coordinate metabolic shifts in metabolome. Atomic Force microscopy will be used to understand the physical properties. New microscopy and bioinformatic tools will be developed to streamline data analysis and organisation.The scientific firsts developed as part of this project will be used exciting research at the forefront of physics biology and chemistry. This will be a valuable resource for researchers to improve their understanding of the physiological state of whole organisms and those furthering the knowledge of C. elegans as a model for complex mammalian biochemistry. This will include optimising disease prediction, diagnosis and intervention.This project will also provide diverse training opportunities for the PhD candidate. With support from the experienced supervisory team. The research conducted will pave-the-way towards establishing new and improved models and analytics for drug delivery to augment understanding of whole organism metabolomics. This will also produce high impact publications and opportunities to disseminate research at international conferences.