Develop a novel in vitro 3D bioelectric system of the GI with enteric nervous system using electronic scaffold devices

本研究的目的是利用电子/光子支架装置，建立人体胃肠道的肠神经模型，研制出一种新型的胃肠道三维生物电系统。因此，允许比目前使用动物模型/啮齿动物细胞的模型更准确的模型。这将允许对生物电系统进行持续的电子监测，从而能够观察药物对肠道疼痛和监测的GI/ENS电性质的影响，以及通过光刺激对模型的刺激。该项目将主要涉及实际的基于实验室的工作，培养不同的细胞培养物，并进一步开发水凝胶支架，以更准确地代表人类胃肠道。实验技术将包括电化学阻抗谱(EIS)、光刺激、跨皮/跨内皮电阻(TEER)和(共聚焦显微镜，扫描电子显微镜)。

This research project aims to produce a novel in vitro 3D bioelectric system of the GI tract with enteric nervous model of the human gastrointestinal track (GI) using electronic/photonic scaffold devices. Thus, allowing for more accurate model than those currently available using animal models/rodent cells. This will allow for continuous electrical monitoring of the bioelectric system, to allow for the observation of the effect of drugs on the electrical nature of GI/ENS over time for enteric pain and monitoring, as well as stimulation of the model through photo -stimulation. This project will mainly involve practical lab-based work growing the different cell cultures and further develop the hydrogel scaffolding to more accurately represent the human GI tract. Experimental techniques will include Electrochemical impedance spectroscopy (EIS), photostimulation, Transepithelial/transendothelial electrical resistance (TEER) and (confocal microscopy, SEM).