Identification of PAS kinase activators

描述(申请人提供)：细胞已经进化出复杂的机制来感知它们的营养环境，并相应地调节生长和增殖。未能适当协调细胞代谢会导致多种人类疾病和综合征。PAS激酶通过感知细胞代谢状态和调节糖代谢，在调节新陈代谢中发挥重要作用。PAS激酶是一种受小分子结合的PAS结构域调节的丝氨酸/苏氨酸蛋白激酶。虽然与生物相关的配体尚不清楚，但已经确定了两种刺激导致酿酒酵母中PAS激酶的激活：第一，在非发酵碳源中的生长；第二，细胞完整性压力。生化和基因技术都将被用来识别参与PAS激酶激活的因素，以响应这两种刺激。这些知识将有助于理解PAS激酶如何协调细胞生长和增殖，以应对环境营养和压力。

DESCRIPTION (provided by applicant): Cells have evolved complex mechanisms to sense their nutritional environment and regulate growth and proliferation accordingly. Failure to properly coordinate cellular metabolism results in a variety of human diseases and syndromes. PAS kinase plays a critical role in coordinating metabolism by sensing cellular metabolic status and regulating sugar metabolism. PAS kinase is a serine/threonine protein kinase regulated in cis by a small molecule-binding PAS domain. Although the biologically relevant ligand is unknown, two stimuli have been identified that lead to activation of PAS kinase in S. cerevisiae: first, growth in non-fermentable carbon sources and, second, cell integrity stress. Both biochemical and genetic techniques will be used to identify factors involved in PAS kinase activation in response to both stimuli. This knowledge will aid in understanding how PAS kinase coordinates cell growth and proliferation in response to environmental nutrients and stresses.