Mechanisms of Olfactory Interneurnon Differentiation

描述（由申请人提供）：本提案的目的是表征源自室管膜下区干细胞龛的哺乳动物成年嗅球中间神经元的基本特征和作用。两个具体的目标已经开发，以确定是否早期出生的中间神经元不同于在成人中产生的，并确定成人衍生的中间神经元的分化活动的影响。具体目标1：为了确定嗅球中间神经元在胚胎和围产期神经元发育过程中的分化是否与成人神经元前体的分化不同，将进行遗传命运作图实验。具体目标二：为了表征活性对成人来源的嗅球中间神经元的影响，将在活性的遗传消融后评估中间神经元形态、基因表达和存活。这些实验将提供重要的信息，成年神经发生的意义和贡献的成熟动物的功能。此外，关于成人神经元分化机制的信息将有助于指导疾病或损伤后神经系统修复的分化。

DESCRIPTION (provided by applicant): The objective of this proposal is to characterize the essential characteristics and role of mammalian, adult-born olfactory bulb interneurons derived from the subventricular zone stem cell niche. Two specific aims have been developed to determine if early-born interneurons differ from those generated in the adult, and to determine the influence of activity on the differentiation of adult-derived interneurons. Specific Aim 1: In order to determine whether olfactory bulb interneurons differentiating during embryonic and perinatal neuronal development are different from those derived from adult neuronal precursors, genetic fate-mapping experiments will be performed. Specific Aim 2: To characterize the influence of activity on adult-derived olfactory bulb interneurons, interneuron morphology, gene expression, and survival will be assessed following genetic ablation of activity. These experiments will contribute important information regarding the significance and contribution of adult neurogenesis to the function of the mature animal. In addition, information regarding the mechanism of neuronal differentiation in the adult will aid in efforts to guide that differentiation toward nervous system repair following disease or injury.