Optimization of Lifetime for Implantable Glucose Sensor

描述(由申请人提供)：迫切需要一种用于糖尿病的新的葡萄糖传感器，可以长期植入组织中。GlySens正在开发一种植入式电化学葡萄糖传感器，该传感器基于固定化的葡萄糖氧化酶和过氧化氢酶，以及可能满足这一需求的恒电位氧传感器。植入物包括传感器、电子和遥测、天线和电池。血糖信号被传输到外部接收器，该接收器将显示实际的血糖值并起到低血糖警报的作用。传感器最终成功的一个关键决定因素是固定化酶的长期稳定性和催化活性，这是本提案的重点。我们已经确定，传感器膜中酶结构域的扩散限制设计和酶的进一步化学稳定将是必要的。我们的工作将包括反应-扩散-失活和两种酶的最佳比例的建模，一种新型聚合物系统的应用和优化，以及加速酶寿命测试。

DESCRIPTION (provided by applicant): There is an urgent need for a new glucose sensor for use in diabetes that can be implanted in tissues for the long term. GlySens is developing an implantable electrochemical glucose sensor based on the immobilized enzymes glucose oxidase and catalase and potentiostatic oxygen sensors that could potentially meet this need. The implant includes the sensor, electronics and telemetry, antenna, and battery. Glucose signals are transmitted to an external receiver that will display the actual glucose value and function as a hypoglycemia alarm. A key determinant of the ultimate success of the sensor is the long-term stability and catalytic activity of the immobilized enzymes, which is the focus of this proposal. We have determined that a combination of diffusion-limited design of enzyme domains in the sensor membrane and further chemical stabilization of the enzymes will be needed. Our efforts will include modeling of reaction-diffusion-inactivation and the optimal ratio of the two enzymes, application and optimization of a novel polymer system, and accelerated enzyme lifetime testing.