MicroArrays in Grass Shrimp and Sheepshead Minnows

描述（由申请人提供）：微阵列是一种测量环境中存在的污染物影响的强大方法。在本提案中，我们将对草虾和羊头鲦鱼的基因进行测序，从这些基因中制作试验微阵列，并显示暴露于环境中存在的污染物所导致的表达模式。对环境中污染物的测量和分析及其对生物群的影响的评估对环境保护署在超级基金监测活动中非常重要草虾和羊头鲦鱼是两个经常用于沿海超级基金监测的物种，它们的各种生理终点已经确定。我们的研究小组和其他人的实验表明，基因阵列可以用来检测暴露于污染物引起的基因表达变化，很明显，污染物具有独特的遗传特征。由于许多污染物在基因水平上通过受体介导和非受体介导的途径诱导或抑制基因表达，微阵列可以帮助阐明受影响的信号通路。

DESCRIPTION (provided by applicant): Microarrays are a powerful way to measure the impact of contaminants present in the environment. In this proposal, we will sequence genes from grass shrimp and sheepshead minnows, make trial microarrays from these genes, and show the expression patterns that result from exposure to contaminants that are present in the environment. Measurement and analysis of contaminants in the environment and evaluation of their effects on biota is very important to the EPA in its Superfund monitoring activities Grass shrimp and sheepshead minnows are two species that are routinely used for monitoring at coastal Superfund sites and for which a variety of physiological endpoints have already been determined. Experiments by our research group and others have shown that gene arrays can be used to detect changes in gene expression caused by exposure to contaminants and it is clear that contaminants have unique genetic signatures. Because many contaminants act at the gene level to induce or repress gene expression through both receptor-mediated and non-receptor mediated pathways, microarrays can help to elucidate signaling pathways that are affected.