CANINE AND FELINE RED CELL ANTIGENS

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。所列机构为中心，不一定是研究者所在机构。溶血性输血反应是人类患者以及接受血液的家畜的显著、严重的并发症。我们与纽约哥伦比亚大学的Steven Spitalnik建立了合作关系，以进一步确定狗和猫的血型系统。 猫的血型只有一个血型系统迄今已被确认的猫。这种猫科动物AB系统由三种血型组成：A型、B型和AB型。A-B不相容性导致急性溶血性输血反应和新生儿溶血。在过去的十年里，我们的合作研究已经很好地描述了遗传、生物化学性质和免疫学后果。此外，我们还协助开发和评估了凝胶测试系统，以准确检测三种血型;原型的初步研究非常令人鼓舞。凝胶测试系统最近已成为人类血型定型的标准方法。 在研究接受血液的动物的相容性的过程中，我们最近发现了一种新的血型抗原。这种抗原被命名为Mik，并且似乎非常常见，因此这可能是一种新的高频红细胞抗原。我们已经证明了在缺乏该抗原的动物中自然产生的针对该抗原的同种抗体的发展。计划进行进一步的研究以更好地确定抗原及其在溶血性输血反应中的作用。 犬的血型已经被研究了几十年，但十几个血型系统的生物化学和分子基础尚未确定。这部分是由于缺乏适当的分型试剂，以及它们在首次输血时明显不重要。尽管如此，我们记录了犬红细胞抗原（DEA）1.1的临床重要性，并使用单克隆抗体对DEA 1.1进行了相同的凝胶试验方法，用于犬的常规筛查。 在这些研究的过程中，我们还认识到一个斑点狗与所有测试的献血者都有泛不相容性。其中包括50只不同品种的狗。然而，当测试这只斑点狗与其他斑点狗时，发现13只中有3只是相容的，这表明它们也缺乏这种红细胞抗原。由于这种抗原在斑点狗中普遍存在且在某些斑点狗中不存在，因此将其命名为Dal，并可能代表一种新的高频红细胞抗原。计划进一步研究以更好地确定抗原及其在溶血性输血反应中的作用。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Hemolytic transfusion reactions are significant, severe complications in human patients as well as domestic animals receiving blood. We have established a collaboration with Steven Spitalnik at Columbia University in New York to further define blood group systems in dogs and cats. Feline Blood Groups Only one blood group system has been thusfar recognized in cats. This feline AB system consists of three blood types: type A, B, and AB. A-B incompatibilities are responsible for acute hemolytic transfusion reactions and hemolysis of the newborn. Our collaborative studies have well characterized the inheritance biochemical nature and immunologic consequences over the past decade. Furthermore, we have assisted in the development and the evaluation of gel test system to detect accurately the three blood types; initial studies on a prototype are very encouraging. Gel test systems have recently becoming standard methods for blood typing in humans. In the process of examining compatibility of animals receiving blood we recently recognized a new blood group antigen. This antigen is named Mik and appears to occur very commonly, hence this may be a novel high frequency red cell antigen. We have documented the development of naturally occurring alloantibodies against this antigen in animals lacking the antigen. Further investigations are planned to better define the antigen and its involvement in hemolytic transfusion reactions. Canine Blood Groups Canine blood groups have been examined for many decades but the biochemical and molecular bases of the more than a dozen blood group systems have not been defined. This is partially due to the lack of appropriate typing reagents and their apparent unimportance at the time of a first transfusion. Nevertheless we documented the clinical importance of the Dog Erythrocyte Antigen (DEA) 1.1 and have applied the same gel test method with a monoclonal antibody to DEA 1.1 for routine screening of dogs. In the process of these studies we also recognized a Dalmatian with a pan-incompatibility to all blood donors tested. This included 50 dogs from various breeds. However, when testing this Dalmatian to other Dalmatians, 3 of 13 were found to be compatible, suggesting that they also lacked this red cell antigen. Because of the common occurrence of this antigen and its absence in some Dalmatians it was named Dal and may represent a new high frequency red cell antigen. Further investigations are planned to better define the antigen and its involvement in hemolytic transfusion rea