NATIONAL RESOURCES FOR APLYSIA

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。描述(由申请人提供)：本提案描述了国家海兔资源的延续，其总体目标是提供持续高质量的养殖海兔(及其养殖的红藻食物Gracilaria sp.)。给NIH赞助的研究人员。海兔是一个重要的非脊椎动物模型系统，主要用于行为和学习的神经生理学方面的健康相关研究。我们将继续生产用于研究的动物，并进行基础研究，旨在探索新的模式用途和改善资源。有四个子项目/具体目标：1.生产-我们预计在下一阶段的资金中，我们的动物产量每年增加约25%，并将以与现场收集的标本具有竞争力的价格提供来自所有生命阶段的标本。2.功能基因组学-我们将对不同发育阶段(幼虫、幼虫、生殖和衰老动物)的神经和其他组织进行cDNA/EST测序项目，并使用这些cDNA来制作微阵列。经过适当的验证和质量控制，这些微阵列将可供我们的用户群以成本价购买，还将用于检测我们的孵化场种群在不同发育阶段的基因表达变化，以及在暴露于其他实验条件(包括可变温度、产蛋激素注射等)后的变化。所有序列和阵列实验数据将通过基于Web的数据库访问提供。3.发育神经生理学-我们将继续研究袋状细胞中的离子电流调制，观察到这些细胞随着发育、遗传背景以及孵化场和野生捕获动物之间的不同而变化。了解生长和成熟的电生理相关性对资源很重要，因为它们可能会影响资源用户对数据的解释。一项相关的研究将检验不同的动物生长速度如何在神经系统变化的发育时间内产生差异，最终达到性成熟。4.动物健康监测-我们将继续实施基于水质和动物健康参数筛选的监测计划，以确保快速检测和完整记录孵化场动物在任何发育阶段可能发生的任何疾病过程。对观察到的任何疾病症状和疑似病原体进行调查，并采取适当的控制措施。通过这种生产与基础研究的结合，完善模型体系，并将其推广到其他研究领域。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. DESCRIPTION (provided by applicant): This proposal describes the continuation of the National Resource for Aplysia, whose overall goal is to provide consistently high-quality cultured sea hares Aplysia californica (and their cultured red algal food, Gracilaria sp.) to NIH-sponsored researchers. Aplysia californica is an important non-vertebrate (Opisthobranch mollusc) model system for health-related research, primarily in the neurophysiology of behavior and learning. We will continue to produce animals for research, and to conduct basic research aimed at exploring new model uses and at improving the resource. There are four sub-projects/specific aims: 1. Production-We anticipate increasing our production of animals by approximately 25% per year during this next phase of funding and will make specimens from all life stages available at a price competitive with fieldcollected specimens. 2. Functional Genomics-We will undertake a cDNA/EST sequencing project of neural and other tissues from different developmental stages (larvae, juveniles, and reproductive and senescent animals), and use these cDNAs to produce microarrays. Following appropriate validation and quality control, these microarrays will be available for at-cost purchase by our user community, and will also be used to examine gene expression changes in our hatchery population during different developmental stages, and after exposure to other experimental conditions including variable temperature, egg-laying hormone administration, etc. All sequence and array experimentation data will be made available through web-based database access. 3. Developmental Neurophysiology- We will continue study of ion current modulation in the bag cells observed to vary with development, genetic background, and between hatchery reared and wild caught animals. Understanding electrophysiological correlates of growth and maturation are important to the Resource because they may affect the interpretation of data by users of the Resource. A correlative study will examine how different growth rates of the animals produce variations in development times for the nervous system changes that culminate in sexual maturity. 4. Animal Health Monitoring-We will continue a monitoring program based on screening of water quality and animal health parameters to assure rapid detection and complete documentation of any disease processes that might occur in any developmental stages of animals at the hatchery. Any disease syndromes and suspected pathogens observed will be investigated and appropriate control measures applied. Through this combination of production and basic research, we will improve the model system and extend its usefullness to other areas of research.