Tiling Path Microarray Re-amplifable Resource Drosophila

描述（由申请人提供）：我们建议为整个常染色质果蝇基因组开发可重复的平铺路径资源。这一资源将用于生产基因组DNA微阵列，以印刷成本分发给社区。这些阵列可用于DNA-蛋白质相互作用作图、综合表达谱、复制起点作图、比较基因组杂交、缺失的物理作图和其他应用。将制定和传播使用这一资源的协议，并将建立一个数据库和数据管理服务，供全基因组平铺路径微阵列用户使用。该资源将通过果蝇基因组学资源中心分发给广大的研究人员。 此外，提出了一种策略，使用新开发的，可寻址系统的可重命名的标签。Ikb PCR产物形式的整个基因组将在四个384孔板中分配给各个实验室。研究人员将能够使用20对独特的引物扩增任何或所有基因组拼接片段。所提出的资源的这一特征将使全面的RNAi筛选、调控序列的快速克隆以及潜在的许多其他应用成为可能。

DESCRIPTION (provided by applicant): We propose to develop a re-amplifiable tiling path resource for the entire euchromatic Drosophila genome. This resource will be used for the production of genomic DNA microarrays to be distributed to the community at cost of printing. These arrays can be used for DNA-protein interaction mapping, comprehensive expression profiling, replication origin mapping, comparative genomic hybridization, physical mapping of deletions, and other applications. Protocols for use with this resource will be developed and disseminated, and a database and data management service will be established for use by users of the whole genome tiling path microarrays. This resource will be distributed to a wide community of researchers through the Drosophila Genomics Resource Center. Additionally, a strategy is proposed to use a newly developed, well-addressable system of re-amplifiable tags. The entire genome in the form of lkb PCR products will be distributable to individual labs in four 384 well plates. A researcher will be able to amplify any or all of the genome tiling fragments using pairs of 20 unique primers. This feature of the proposed resource would enable comprehensive RNAi screens, rapid cloning of regulatory sequences, and potentially many other applications.