Illumina Genome AnalyzerII (GAII) (110v/220v)

描述(由申请人提供)：我们请求Illumina Solexa GAIIx系统，这将是NIH资助的大芝加哥大都市区范围广泛的项目的一个必不可少的“下一代”测序平台。这台新机器将安装在芝加哥大学基因组和系统生物学联合研究所的高通量基因组分析核心(HGAC)和阿贡国家实验室(http://www.igsb.org/services/index.php?p=hgac.)重要的是，这一能力的增加将使洛约拉大学和芝加哥伊利诺伊大学等大学的NIH资助的研究人员能够获得最先进的技术。芝加哥大学和西北大学的研究人员已经利用了现有的设施，但我们的能力有限，因为NIH资助的主要赠款，如NHGRI modENCODE项目(U01HG004264)已经以100%的能力占用了现有的机器(两台Solexa GAII机器和一台罗氏GS-FLX 454测序仪)。具体地说，来自芝加哥大学癌症中心的多名NIH资助的研究人员，他们是IGSB领导的“1000芝加哥癌症转录本倡议”的一部分，他们将通过所请求的仪器获得支持。此外，芝加哥系统生物学中心(CCSB)的研究人员将得到该仪器的支持，CCSB是由NIGMS资助的十个国家系统生物学中心之一。因此，资助这项仪器申请将不仅利用现有的HGAC基础设施，而且还将利用NIH资助的多个项目，这些项目由芝加哥地区数十名研究人员资助，他们需要全基因组测序和重测序、转录组测序、从头测序、SNP发现、基因表达、小RNA发现或芯片序列。这台机器对于成功和立竿见影至关重要，将与现有的生产服务管道整合，其中包括训练有素的技术人员，他们在核酸分离、图书馆建设、集群生成和Solexa的质量控制方面都有经验；以及在过去18个月里与IGSB(www.igsb.org)的一组生物信息学专家一起开发的LIMS、计算、初步分析和数据分发基础设施。这将使该机器对芝加哥四所大学的NIH调查人员产生立竿见影的影响。

DESCRIPTION (provided by applicant): We request the Illumina Solexa GAIIx system, which will be an essential "next generation" sequencing platform for a wide range of NIH funded projects at the greater Chicago metropolitan area. This new machine will be housed at the High-Throughput Genomic Analysis Core (HGAC) of the Joint Institute of Genomics and Systems Biology (IGSB) at the University of Chicago and Argonne National Laboratory (ANL) http://www.igsb.org/services/index.php?p=hgac. Importantly, the addition of this capacity will enable NIH funded investigators at universities such as Loyola University and University of Illinois at Chicago to gain access to state-of-the-art technology. Investigators at The University of Chicago and Northwestern University already make use of existing facility, but our capacity is limited due to major NIH funded grants such as an NHGRI modENCODE project (U01HG004264) already occupying existing machines at 100% capacity (two Solexa GAII machines and one Roche GS-FLX 454 sequencer). Specifically, multiple NIH funded investigators from the University of Chicago Cancer Center and who are part of the IGSB-led "1000 Chicago Cancer Transcriptomes Initiative" will be enabled by the requested instrument. Also, investigators in the Chicago Center for Systems Biology (CCSB), one of ten National Centers for Systems Biology funded by NIGMS, would be supported with this instrument. Funding this instrument request would thus leverage not only the existing HGAC infrastructure, but also multiple NIH research funded projects of dozens of investigators in the Chicago area who require whole-genome sequencing and re-sequencing, transcriptome sequencing, de novo sequencing, SNP discovery, gene expression, small RNA discovery, or ChIP-seq. Critically important for success and immediate impact, the machine would be integrated with an existing production service pipeline that includes trained technical staff with experience in all aspects of nucleic acid isolation, library construction, cluster generation and quality control for Solexa; as well as a LIMS, computing, primary analysis and data distribution infrastructure that has been developed with a team of bioinformatics experts in IGSB (www.igsb.org) over the last 18 months. This will allow the machine to have immediate impact on NIH investigators at four universities in Chicago.