Illumina Genome AnalyzerII (GAII) (110v/220v)

描述（由申请人提供）：我们要求Illumina Solexa GAIIx系统，这将是一个重要的“下一代”测序平台，用于在大芝加哥大都会地区广泛的NIH资助项目。这台新机器将被安置在芝加哥大学基因组学和系统生物学联合研究所（IGSB）和阿贡国家实验室（ANL） http://www.igsb.org/services/index.php?p=hgac的高通量基因组分析核心（HGAC）中。重要的是，这种能力的增加将使美国国立卫生研究院资助的洛约拉大学和伊利诺伊大学芝加哥分校等大学的研究人员能够获得最先进的技术。芝加哥大学和西北大学的研究人员已经利用了现有的设备，但由于NIH的主要资助，我们的能力有限，例如NHGRI modENCODE项目（U01HG004264）已经占用了现有的机器100%的容量（两台Solexa GAII机器和一台罗氏GS-FLX 454测序仪）。具体来说，来自芝加哥大学癌症中心的多名NIH资助的研究人员，他们是igsb领导的“1000个芝加哥癌症转录组计划”的一部分，将通过所要求的仪器启用。此外，由NIGMS资助的十个国家系统生物学中心之一的芝加哥系统生物学中心（CCSB）的研究人员将得到该仪器的支持。因此，资助这一仪器申请不仅将利用现有的HGAC基础设施，而且还将利用芝加哥地区数十名研究人员资助的多个NIH研究项目，这些研究人员需要全基因组测序和重测序、转录组测序、从头测序、SNP发现、基因表达、小RNA发现或ChIP-seq。对于成功和直接影响至关重要的是，该机器将与现有的生产服务管道集成，其中包括在核酸分离，库构建，集群生成和Solexa质量控制的各个方面具有经验的训练有素的技术人员；以及由IGSB （www.igsb.org）的一组生物信息学专家在过去18个月里开发的LIMS、计算、初级分析和数据分发基础设施。这将使这台机器对美国国立卫生研究院在芝加哥四所大学的研究人员产生直接影响。

DESCRIPTION (provided by applicant): We request the Illumina Solexa GAIIx system, which will be an essential "next generation" sequencing platform for a wide range of NIH funded projects at the greater Chicago metropolitan area. This new machine will be housed at the High-Throughput Genomic Analysis Core (HGAC) of the Joint Institute of Genomics and Systems Biology (IGSB) at the University of Chicago and Argonne National Laboratory (ANL) http://www.igsb.org/services/index.php?p=hgac. Importantly, the addition of this capacity will enable NIH funded investigators at universities such as Loyola University and University of Illinois at Chicago to gain access to state-of-the-art technology. Investigators at The University of Chicago and Northwestern University already make use of existing facility, but our capacity is limited due to major NIH funded grants such as an NHGRI modENCODE project (U01HG004264) already occupying existing machines at 100% capacity (two Solexa GAII machines and one Roche GS-FLX 454 sequencer). Specifically, multiple NIH funded investigators from the University of Chicago Cancer Center and who are part of the IGSB-led "1000 Chicago Cancer Transcriptomes Initiative" will be enabled by the requested instrument. Also, investigators in the Chicago Center for Systems Biology (CCSB), one of ten National Centers for Systems Biology funded by NIGMS, would be supported with this instrument. Funding this instrument request would thus leverage not only the existing HGAC infrastructure, but also multiple NIH research funded projects of dozens of investigators in the Chicago area who require whole-genome sequencing and re-sequencing, transcriptome sequencing, de novo sequencing, SNP discovery, gene expression, small RNA discovery, or ChIP-seq. Critically important for success and immediate impact, the machine would be integrated with an existing production service pipeline that includes trained technical staff with experience in all aspects of nucleic acid isolation, library construction, cluster generation and quality control for Solexa; as well as a LIMS, computing, primary analysis and data distribution infrastructure that has been developed with a team of bioinformatics experts in IGSB (www.igsb.org) over the last 18 months. This will allow the machine to have immediate impact on NIH investigators at four universities in Chicago.