Illumina Genome AnalyzerII (GAII) (110v/220v)

描述（由申请人提供）：我们请求Illumina Solexa GAIIx系统，该系统将成为大芝加哥大都市区NIH资助的广泛项目的重要“下一代”测序平台。这台新机器将被安置在芝加哥大学和阿贡国家实验室（ANL）基因组学和系统生物学联合研究所（IGSB）的高通量基因组分析核心（HGAC）http://www.igsb.org/services/index.php? p=hgac。重要的是，这种能力的增加将使美国国立卫生研究院资助的研究人员在大学，如洛约拉大学和伊利诺伊大学芝加哥获得国家的最先进的技术。芝加哥大学和西北大学的研究人员已经利用了现有的设施，但由于NIH的主要赠款例如NHGRI modENCODE项目（U 01 HG 004264）已经占用了100%的现有机器（两台Solexa GAII机器和一台Roche GS-FLX 454测序仪），我们的能力有限。具体而言，来自芝加哥大学癌症中心的多名NIH资助的研究人员以及IGSB领导的“1000个芝加哥癌症转录组计划”的一部分将通过所请求的仪器启用。此外，芝加哥系统生物学中心（CCSB）的研究人员，NIGMS资助的十个国家系统生物学中心之一，将得到这种仪器的支持。因此，为该仪器请求提供资金不仅可以利用现有的HGAC基础设施，还可以利用芝加哥地区数十名研究人员的多个NIH研究资助项目，这些研究人员需要全基因组测序和重测序、转录组测序、从头测序、SNP发现、基因表达、小RNA发现或ChIP-seq。对于成功和直接影响至关重要的是，该机器将与现有的生产服务管道集成，其中包括训练有素的技术人员，他们在核酸分离，文库构建，集群生成和Solexa质量控制的各个方面都有经验;以及LIMS，计算，主要分析和数据分发基础设施，这些基础设施是在过去18个月内与IGSB（www.igsb.org）的生物信息学专家团队一起开发的。这将使该机器对芝加哥四所大学的NIH调查人员产生直接影响。

DESCRIPTION (provided by applicant): We request the Illumina Solexa GAIIx system, which will be an essential "next generation" sequencing platform for a wide range of NIH funded projects at the greater Chicago metropolitan area. This new machine will be housed at the High-Throughput Genomic Analysis Core (HGAC) of the Joint Institute of Genomics and Systems Biology (IGSB) at the University of Chicago and Argonne National Laboratory (ANL) http://www.igsb.org/services/index.php?p=hgac. Importantly, the addition of this capacity will enable NIH funded investigators at universities such as Loyola University and University of Illinois at Chicago to gain access to state-of-the-art technology. Investigators at The University of Chicago and Northwestern University already make use of existing facility, but our capacity is limited due to major NIH funded grants such as an NHGRI modENCODE project (U01HG004264) already occupying existing machines at 100% capacity (two Solexa GAII machines and one Roche GS-FLX 454 sequencer). Specifically, multiple NIH funded investigators from the University of Chicago Cancer Center and who are part of the IGSB-led "1000 Chicago Cancer Transcriptomes Initiative" will be enabled by the requested instrument. Also, investigators in the Chicago Center for Systems Biology (CCSB), one of ten National Centers for Systems Biology funded by NIGMS, would be supported with this instrument. Funding this instrument request would thus leverage not only the existing HGAC infrastructure, but also multiple NIH research funded projects of dozens of investigators in the Chicago area who require whole-genome sequencing and re-sequencing, transcriptome sequencing, de novo sequencing, SNP discovery, gene expression, small RNA discovery, or ChIP-seq. Critically important for success and immediate impact, the machine would be integrated with an existing production service pipeline that includes trained technical staff with experience in all aspects of nucleic acid isolation, library construction, cluster generation and quality control for Solexa; as well as a LIMS, computing, primary analysis and data distribution infrastructure that has been developed with a team of bioinformatics experts in IGSB (www.igsb.org) over the last 18 months. This will allow the machine to have immediate impact on NIH investigators at four universities in Chicago.