Core--Outcome Measures and Cell Preparation

核心B提供用于治疗的细胞，以及该计划项目所有组成项目的基本结果测量。神经功能和行为是项目1（用间充质干细胞治疗中风）项目2（用间充质干细胞治疗创伤性脑损伤）的主要结果测量指标，并广泛应用于项目3 （MSC与组织相互作用的分析）。在本核心中，我们描述了用于评估骨髓间充质干细胞治疗反应的功能测试集。包括一系列测试，运动和体感测试，以及神经系统测试。项目1-3还讨论了注射的间充质干细胞的命运、这些细胞产生的因子及其对脑组织促进功能改善的影响，以及间充质干细胞对内源性实质细胞的影响。该核心提供了细胞鉴定，营养因子的测量和鉴定以及显微镜所需的所有资源。这三个项目都使用骨髓基质细胞。核心B，提供专门生产和表征这些细胞的设施和人员。将核心B中组成项目所需的资源集中起来，可以有效地利用所有三个项目所需的资源。

Core B provides the cells to be employed for treatment as well as the essential outcome measurements for all constituent projects of the Program Project. Neurologic function and behavior are the primary outcome measures for Project 1 (Treatment of Stroke with MSCs) Project 2 (Treatment of Traumatic Brain Injury with MSCs) and are extensively employed in Project 3 (Analysis of MSC Interaction with Tissue). In this core, we describe the sets of functional tests to be employed in evaluating response to MSC therapy. Included in the battery of tests are, motor and somatosensory tests, and neurological scales. Projects 1-3 also address the fate of the injected MSCs, the factors produced by these cells and their effect on cerebral tissue that promote improved functional outcome, and the effects of MSCs on the endogenous parenchymal cells. This core provides all the resources required for cellular identification, measurement and identification of trophic factors, and microscopy. All three projects employ marrow stromal cells. Core B, provides the facilities and personnel dedicated to the production and characterization of these cells. Focusing resources needed for the component projects in Core B, allows for efficient and effective utilization of resources essential for all three projects.