Behavioral actions of angiotensin II: Circuits and intracellular signals

描述（由申请人提供）： 项目概要：候选人、环境和研究。该提案描述了一个为期5年的培训计划，旨在为候选人提供实现其长期目标所需的专业知识，即在一所学术研究型大学发展独立的行为神经科学家职业生涯。候选人获得了博士学位。2001年，他在宾夕法尼亚大学动物生物学系和马奥尼神经科学研究所担任博士后研究员。马奥尼神经科学研究所拥有来自宾夕法尼亚大学18个部门和6所学校的180多名教职员工，并为候选人提供了理想的培训环境。Steven J. Fluharty博士是行为神经科学领域的知名研究人员，也是对拟议实验中研究的激素（强效dipsogen血管紧张素II（AngII））的细胞和行为反应的专家。研究计划的重点是水，盐和食物的摄入量，体液，心血管和能量稳态的关键组成部分。将这些行为理解为独立但相关的摄食行为形式，有可能揭示对其控制和调节的新见解。这里描述的实验旨在确定与摄食行为的激素刺激相关的细胞内事件。AngII受体产生多种细胞内反应，但这些反应与AngII治疗产生的行为之间的联系仍不清楚。本文所述的实验利用新的药理学方法来确定这些不同的细胞内反应对AngII诱导的摄食行为的行为相关性，并探索一般摄食行为的共性。 项目摘要：与公共卫生的相关性。水、盐和食物的摄入对于维持体内平衡至关重要。影响体液、心血管和能量稳态的疾病包括高血压、心脏或肾脏疾病、糖尿病和肥胖症。确定激素介导这些行为的神经机制对于完全理解这些现象至关重要，并可能导致新的治疗方法来对抗相关疾病状态。

DESCRIPTION (provided by applicant): Project summary: candidate, environment, and research. This proposal describes a 5-year training program designed to provide the candidate with the expertise needed to achieve his long-term goal of developing a career as an independent behavioral neuroscientist at an academic research university. The candidate received a Ph.D. in 2001 and has since been conducting research as a postdoctoral fellow in the department of Animal Biology and the Mahoney Institute for Neurological Sciences at the University of Pennsylvania. The Mahoney Institute for Neurological Sciences houses over 180 faculty members from eighteen departments and six schools within the University of Pennsylvania and provides an ideal training environment for the candidate. The proposed mentor, Dr. Steven J. Fluharty, is a well-established researcher in the field of behavioral neuroscience and an expert in the cellular and behavioral responses to the hormone studied in proposed experiments, the potent dipsogen angiotensin II (AngII). The research plan focuses on water, salt, and food intake, key components of body fluid, cardiovascular, and energy homeostasis. Understanding these behaviors as separate, but related forms of ingestive behavior has the potential to reveal novel insights into their control and regulation. The experiments described here are designed to determine the intracellular events associated with the hormonal stimuli for ingestive behavior. AngII receptors produce a diverse array of intracellular responses, yet the connection between any of these responses and the behaviors produced by treatment with AngII remains unclear. The experiments described here take advantage of novel pharmacological approached to determine the behavioral relevance of these divergent intracellular responses to AngII-induced ingestive behaviors and explore commonalities of ingestive behaviors in general. Project summary: Relevance to public health. Ingestion of water, salt, and food is critical for maintenance of homeostasis. Disorders affecting body fluid, cardiovascular, and energy homeostasis include hypertension, heart or kidney disease, diabetes, and obesity. Determining the neural mechanisms through which hormones mediate these behaviors is critical for a complete understanding of the phenomena and may lead to novel therapeutic approaches to combat the relevant disease states.