Mitogen activated protein kinases regulation-PTH/PTHrP

描述(由申请人提供)： 这项K01建议的最终目的是为申请者提供额外的基础科学和分子生物学方面的培训，以确立他作为一名独立科学家的地位。该项目阐明了PTH/PTHrP受体调节细胞外信号调节(ERK)丝裂原活化蛋白(MAP)激酶的分子机制和功能。非受体酪氨酸激酶的作用以及受体磷酸化在成骨细胞系中PTH激活ERK1/2 MAP激酶中的作用将被探索。此外，还将研究ERK1/2 MAP激酶在成骨细胞增殖、分化和凋亡中的作用。来自野生型和基因工程小鼠的原代成骨细胞培养以及其他几种生化和药理学工具将被用于实现研究的目标。

DESCRIPTION (provided by applicant): The ultimate goal of this K01 proposal is to provide the applicant with additional training in basic science and molecular biology to establish himself as an independent scientist. The project elucidates the molecular mechanisms and the function of PTH/PTHrP receptor regulation of Extracellular signal regulated (ERK) mitogen-activated protein (MAP) kinases. The role of non-receptor tyrosine kinases as well as the role of receptor phosphorylation in PTH activation of ERK1/2 MAP kinases in osteoblastic cell lines will be explored. Furthermore, the role of ERK1/2 MAP kinases in osteoblastic cell proliferation, differentiation and apoptosis will be investigated. Primary osteoblastic cell culture from wild type and genetically engineered mice as well as several other biochemical and pharmacological tools will be employed to achieve the goals of the study.