Mitogen activated protein kinases regulation-PTH/PTHrP

本K 01提案的最终目标是为申请人提供基础科学方面的额外培训， 分子生物学，以确立自己作为一个独立的科学家。该项目阐明了分子 PTH/PTHrP受体对细胞外信号调节蛋白（ERK）的调节作用及其机制 丝裂原活化蛋白（MAP）激酶。非受体酪氨酸激酶的作用以及 将探讨成骨细胞系中PTH激活ERK 1/2 MAP激酶的受体磷酸化。 此外，ERK 1/2 MAP激酶在成骨细胞增殖、分化和凋亡中的作用， 将被调查。野生型和基因工程小鼠的原代成骨细胞培养 因为将使用几种其它生物化学和药理学工具来实现研究的目标。

The ultimate goal of this K01 proposal is to provide the applicant with additional training in basic science and molecular biology to establish himself as an independent scientist. The project elucidates the molecular mechanisms and the function of PTH/PTHrP receptor regulation of Extracellular signal regulated (ERK) mitogen-activated protein (MAP) kinases. The role of non-receptor tyrosine kinases as well as the role of receptor phosphorylation in PTH activation of ERK1/2 MAP kinases in osteoblastic cell lines will be explored. Furthermore, the role of ERK1/2 MAP kinases in osteoblastic cell proliferation, differentiation and apoptosis will be investigated. Primary osteoblastic cell culture from wild type and genetically engineered mice as well as several other biochemical and pharmacological tools will be employed to achieve the goals of the study.