INFRASTRUCTURE 1: CORE RESEARCH LABORATORIES

本子项目是利用由NIH/NCRR资助的中心赠款提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是中心的，不一定是研究者的机构。CBR/RCMI核心设施加强了塔斯基吉大学研究人员在显微镜/成像，流式细胞术，细胞培养和分子生物学以及生物信息学和计算生物学方面的多学科研究。今年，核心设施为代表3个学院和9个学科的16个用户提供了服务和帮助。发表或提交同行评议论文5篇，发表论文或海报9篇。成像设施继续为最多的研究人员提供服务，而细胞培养和分子生物学设施积累了最多的“工时”使用。最近，通过C Imaging Systems Simple PCI软件操作的Q Imaging CE数码相机被添加到光成像设施中。该相机极大地增强了该设施的荧光显微镜功能，并允许在不使用放射底片的情况下记录化学发光凝胶。飞利浦400 TEM配备了一个科学仪器与应用（SIA） Pelter冷却CCD相机（320万像素）；使用SIA软件包捕获的图像（TIFF文件）进行处理和分析。通过购买几个最先进的仪器，分子生物学能力得到了显著增强。其中包括BIO-TEK SYNERGY HT微孔板读取器，可读取6至384孔板，并进行荧光，吸光度和发光测量。添加了一个epppendorf Mastercycler，更新了传统的PCR。已经购买了造父变星Smartcycler 2，提供出色的实时PCR功能。仪器采用4个光通道；发射范围从510纳米到750纳米。这些通道允许同时检测四个“探针”。该仪器提供生长曲线、温度曲线和熔化曲线的实时显示，并进行定性、定量和生长曲线分析。生物信息学和计算生物学设施的目标是通过使用基于网络的基础设施，特别是在基因组学和蛋白质组学方面，增加教师对生物信息学和计算生物学研究的参与。我们已经添加了Accelrys的更新软件，并继续与阿拉巴马大学伯明翰基因组核心设施的合作。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The CBR/RCMI Core Facility enhances multidisciplinary research of Tuskegee University investigators in Microscopy/Imaging, Flow Cytometry, Cell Culture and Molecular Biology, and Bioinformatics and Computational Biology. This year, the Core Facility has provided service and assistance to 16 users representing 3 academic colleges and 9 scientific disciplines. These investigators published or submitted 5 peer reviewed papers and presented 9 papers or posters. The Imaging Facility continues to offer service to the largest number of investigators while the Cell Culture and Molecular Biology facilities accumulated the most "man hours" of use. . A Q Imaging CE digital camera operating through C Imaging Systems Simple PCI software was recently added to the light imaging facility. This camera greatly enhances the fluorescent microscopy capabilities of the facility and should allow the recording of chemoluminescent gels without the use of radiographic film. The Philips 400 TEM was equipped with a Scientific Instruments & Applications (SIA) Pelter Cooled CCD Camera (3.2 megapixels); images captured using the SIA software package (TIFF files) are processed and analyzed. Molecular biology capabilities have been significantly enhanced through the purchase of several state of the art instruments. These include a BIO-TEK SYNERGY HT microplate reader, which reads 6-to-384 well plates and performs fluorescence, absorbance and luminescence measurements. An Eppendorf Mastercycler has been added updating conventional PCR. A Cepheid Smartcycler 2 has been purchased and provides outstanding Real-time PCR capabilities. The instrument employs 4 optical channels; emissions range from 510 nm to 750 nm. These channels permit simultaneous detection of four "probes". The instrument provides real-time display of growth curves, temperature profiles, and melting curves with qualitative, quantitative, and growth curve analysis. The goal of the Bioinformatics and Computational Biology Facility is to increase participation of faculty in bioinformatics and computational biology research through the use of web based infrastructure, especially in genomics and proteomics. We have added updated software from Accelrys and are continuing our association with the University of Alabama at Birmingham Genomics Core Facility.