Effect of Neonatal Insult on Adult Visceral Afferents

描述（由申请人提供）：新生儿早期发育期间的炎症或疼痛会导致伤害性加工的长期结构和功能改变。虽然这一现象在体感觉系统中得到了广泛的研究，但新生儿炎症对脏器感觉系统的影响相对未知。这一建议的目的是解决新生儿结肠炎症对成年小鼠内脏疼痛处理的长期影响。小鼠新生儿结肠炎症模型将用于确定是否可以诱导内脏传入神经的解剖和生理特性发生永久性变化，导致它们永久致敏，从而建立慢性内脏痛觉过敏状态。结合解剖学和生理学技术，将研究1)结肠传入神经的中枢和外周终止模式和脊髓组织的变化，2)对有害刺激的有害反应，3)结肠传入神经的电生理反应模式。这里获得的数据将为新生儿时期脏器感觉系统的可塑性提供重要信息。

DESCRIPTION (provided by applicant): Inflammation or pain during early neonatal development results in long-term structural and functional alterations in nociceptive processing. While this phenomenon has been extensively studied within the somatosensory system, the impact of neonatal inflammation on the viscerosensory system is relatively unknown. This proposal has been designed to address the long-term effects of neonatal colon inflammation on the processing of visceral pain in adult mice. A model of neonatal colonic inflammation in mice will be used to determine if permanent changes can be induced in anatomical and physiological properties of visceral afferents, causing them to become permanently sensitized and thereby establishing a state of chronic visceral hyperalgesia. Using a combination of anatomical and physiological techniques, changes will be investigated in 1) the central and peripheral termination patterns and spinal organization of colonic afferents, 2) nocifensive responses to noxious stimuli and 3) the electrophysiological response patterns of colonic afferents. The data obtained here will provide important information regarding the plasticity of the viscerosensory system during neonatal periods.