Learning & memory dependent encoding in the hippocampus

描述(申请人提供)：记忆对身份和身份至关重要 海马体是正常记忆所必需的。因果关系 海马神经元活动与学习记忆的关系 然而，性能仍然未知，因为很少有实验记录神经元 在内存需求变化且保持行为的其他方面时触发 常量。目的1.通过以下方式识别神经元编码中的学习依赖性变化 记录大鼠执行一项任务时的海马神经元组 海马体。成功的新学习需要海马体 需要通过海马区形成新的和持续的放电模式 神经元。老鼠将接受+迷宫任务的训练，这项任务可以由任何一个人来解决 依赖于海马体或独立的策略。海马区细胞群 将记录在行为相同但学习能力不同的大鼠身上 能力、记忆策略和海马体功能。探测器将测试 每只大鼠使用的策略和单位活动将在正常情况下进行比较 和反应学习者，以及穹隆或尾状损害的大鼠。目标2.测试 目标1中结果的一般性，神经元活动将记录在 大鼠接受了一种独特类型的学习，社会传递食物 偏好。可能需要一个动态神经元反应的子集 与行为表达无关的海马体依赖学习的类型， 学习动机的类型和具体的学习内容。大鼠海马神经元 正常大鼠应该发展出持续的嗅觉联系 随意选择新的食物气味和二硫化碳的气味， 食品安全的信号。目的3.检验脉冲之间的时间关系 训练通过比较实时集合活动来编码记忆 海马体。重要的存储器信息可以由临时的 神经元动作电位之间的关系。扣球的时间动力学 在相同的行为但不同的记忆负荷期间记录的合奏中 将使用已公布的和一种新的方法进行评估。分析将从以下方面开始 比较大鼠右横穿迷宫起始臂时的记录 然后左转。

DESCRIPTION (provided by applicant): Memory is crucial to identity and survival, and the hippocampus is required for normal memory. The causal relationship between hippocampal cell activity and learning and memory performance, however, remains unknown because few experiments record neuronal firing while memory demands are varied and other aspects of behavior are held constant. Aim 1. To identify learning-dependent changes in neuronal encoding by recording groups of hippocampal neurons while rats perform a task that requires the hippocampus. Successful newlearning that requires the hippocampus should require the formation of new and persistent firing patterns by hippocampal neurons. Rats will be trained in a + maze task that can be solved by either hippocampus-dependent or independent strategies. Groups of hippocampal cells will be recorded in rats that behave identically but differ in learning ability, memory strategy, and hippocampal function. Probes will test the strategies used by each rat, and unit activity will be compared in normal place and response learners, and rats with fornix or caudate lesions. Aim 2. To test the generality of the results in Aim 1, neuronal activity will be recorded in rats trained in a distinct type of learning, the social transmission of food preference. A subset of dynamic neuronal responses may be required for all types of hippocampus-dependent learning, independent of behavioral expression, type of motivation, and specific content of learning. Hippocampal neurons in normal rats should develop persistent olfactory associations between the arbitrarily selected novel food odors and the odor of carbon disulfide, the signal of food safety. Aim 3. To test if temporal relationships among spike trains encode memory by comparing real-time ensemble activity in the hippocampus. Significant memory information may be encoded by the temporal relationships among neurons action potentials. The temporal dynamics of spiking within ensembles recorded during identical behaviors but different memoryloads will be assessed using published and a new method. The analysis will begin by comparing spike records as rats traverse the start arm of a + maze during right and left turns.