Understanding effects of formulations on ingredient interaction with tissue culture models of the oral epithelium

本项目将研究组织工程口腔粘膜等效物的建立和验证，作为一种高通量筛选工具，用于维持和保护口腔健康的产品中选定的活性物质和赋形剂。具体来说，我们的目标是(i)研究口腔粘膜等效物的屏障特性（ii）包括氯己定、CPC、口服等效物和猪口腔组织中的cpc -锌和锌-异丙基甲基苯酚（iii）口服保健品中常用的某些赋形剂的吸收如何影响这些活性的渗透（iv）为了使口服等效物中活性的最佳递送，是否可以修改赋形剂的性质和数量(v)口服组织等效物预测活性和赋形剂在猪口腔组织中的吸收的能力。口腔组织等效模型的建立与表征使用常规分析方法（GC， HPLC， NMR）火焰吸收原子光谱以及包括共聚焦拉曼光谱在内的先进技术表征口服组织等效物的活性和赋形剂渗透。3. 本项目将建立口腔上皮的新模型，用于快速开发和筛选用于口腔健康管理的配方，并将引起学术界和工业界的极大兴趣。

This project will investigate the establishment and validation of tissue engineered oral mucosal equivalents as a high throughput screening tool for selected actives and excipients used in products to maintain and protect oral health.Specifically, we aim to(i) Investigate the barrier properties of oral mucosal equivalents (ii) Penetration and uptake of actives including chlorhexidine, CPC, CPC-zinc and zinc-isopropylmethyl phenol in oral equivalents and in porcine buccal tissue (iii) How the uptake of selected excipients commonly used in oral healthcare products influences the penetration of these actives (iv) If the nature of the excipients and quantities may be modified for optimal delivery of actives in oral equivalents (v)The capability of oral tissue equivalents to predict uptake of actives and excipients in porcine buccal tissue Objectives: 1. Establishment and characterisation of oral tissue equivalent models 2. Characterisation of active and excipient permeation in oral tissue equivalents using conventional analytical methods (GC, HPLC, NMR) flame absorption atomic spectroscopy as well as advanced techniques including confocal Raman spectroscopy. 3. Evaluate efficacy of oral tissue equivalents for engineering novel formulations for oral health conditions This project will establish new models of the oral epithelium for rapid development and screening of formulations for management of oral health and will be of considerable interest to academia and industry.