METAPNEUMOVIRUS INFECTIONS OF CHILDREN IN INDIA

描述（由研究者提供）：人偏肺病毒（hMPV）是一种新描述的急性呼吸道感染（ARI）病毒病原体。 没有关于hMPV在印度或任何其他发展中国家的作用的描述。拟议的工作将检验hMPV是印度ARI负担的重要贡献者的假设。印度每年有250万5岁以下儿童死亡。急性呼吸道感染导致多达50万人死亡。拟定研究为探索性研究，因为没有关于hMPV对印度ARI贡献的数据。对这些研究的支持将提高世界第二人口大国研究hMPV的能力。 目标1.确定发展中国家印度与hMPV相关的ARI比例。在新德里的一家大型城市教学医院就诊的ARI和ALRI儿童将入选本研究。将描述疾病的临床谱。将通过RT-PCR和细胞培养检测鼻咽样本中是否存在hMPV。 目标2.开发印度hMPV的分子检测和遗传特性鉴定技术。 采用实时荧光定量RT-PCR检测方法，提高hMPV检测的灵敏度。将确定hMPV的N、F和G基因区域的核苷酸序列，并在印度hMPV和其他国家的病毒之间进行比较。这些数据将有助于确定hMPV的全球流行病学，并为印度的hMPV诊断检测和疫苗开发提供关键信息。 目标3.确定印度一家大型城市教学医院不同年龄组中hMPV抗体的流行率。没有关于发展中国家hPMV抗体流行率的数据。将开发用于检测hMPV抗体的检测方法并定义血清阳性率。

DESCRIPTION (provided by investigator): Human metapneumovirus (hMPV) is a newly described viral pathogen of acute respiratory infection (ARI). There are no descriptions of the role of hMPV in India or any other developing country. The proposed work will test the hypothesis that hMPV is an important contributor to the burden of ARI in India. India has 2.5 million deaths per year among children less than 5 years of age. ARI causes as many as 0.5 million of these deaths. The proposed studies are exploratory as there are no data on the contribution of hMPV to ARI in India. Support for these investigations will develop capacity for the study of hMPV in the second most populous country in the world. Aim 1. Determine the proportion of ARI associated with hMPV in a developing country, India. Children with ARI and ALRI seen in a large urban teaching hospital in New Delhi will be enrolled in the study. The clinical spectrum of disease will be described. Nasopharyngeal samples will be tested for the presence of hMPV by RT-PCR and cell culture. Aim 2. Develop techniques for molecular detection and genetic characterization of hMPV from India. Real-time RT-PCR detection will be used to improve the sensitivity of detection of hMPV. Nucleotide sequences for regions of the N, F, and G genes of hMPV will be determined and compared among Indian hMPV and viruses from other countries. These data for will help define the global epidemiology of hMPV and provide critical information relevant to hMPV diagnostic assay and vaccine development for India. Aim 3. Determine the prevalence of antibodies to hMPV among different age groups at a large urban teaching hospital in India. No data are available as to prevalence of antibodies to hPMV in developing countries. Assays for detection of hMPV antibodies will be developed and seroprevalence defined.