Detection and genetic diversity of human metapneumovirus in hospitalized children with acute respiratory infections in India.
Detection and genetic diversity of human metapneumovirus in hospitalized children with acute respiratory infections in India.
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DOI：
10.1002/jmv.22176
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发表时间：
2011-10
影响因子：
12.7
通讯作者：
Broor, Shobha
中科院分区：
文献类型：
--
作者：
Banerjee, Sagarika;Sullender, Wayne M.;Choudekar, Avinash;John, Cherian;Tyagi, Vikas;Fowler, Karen;Lefkowitz, Elliot J.;Broor, Shobha
Human metapneumovirus (hMPV) causes acute respiratory infections in children and adults. It is classified into two major genetic lineages and each lineage into two sublineages. The purpose of the study was to identify and characterize hMPV in children who presented to the All India Institute of Medical Sciences, New Delhi, India with acute respiratory infection from April 2005 to March 2007. By reverse-transcription polymerase chain reaction, hMPV was detected in 21 (3%) of the 662 nasopharyngeal samples from children with acute respiratory infection and in none of the 120 control children. Seven of the 21 (33%) children infected with hMPV required hospital admission for pneumonia or bronchiolitis. Most hMPV detections were during the winter and spring seasons. The majority (67%, 11/21) of children positive for hMPV were within 24 months of age. Phylogenetic analysis of partial F and N gene and the full G gene sequences showed three sub-lineages of hMPV circulated during the study period, B1, B2 and the novel sub-lineage A2b. The circulation pattern of hMPV genotypes varied by season. Comparison of the F and G genes of 8 strains revealed incongruencies in lineage assignments, raising the possibility that recombination had occurred. Sequence analysis also revealed the F gene was relatively conserved whereas the G gene was more variable between the A and B lineages. This study demonstrates that hMPV is an important contributor to acute respiratory infection in children in India, resulting in both outpatient visits and hospitalizations.
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DOI：
10.1016/s0140-6736(02)11391-2
发表时间：
2002-11-02
期刊：
Lancet (London, England)
影响因子：
--
作者：
Jartti T;van den Hoogen B;Garofalo RP;Osterhaus AD;Ruuskanen O
通讯作者：
Ruuskanen O
影响因子：
11.8
作者：
Huck B;Scharf G;Neumann-Haefelin D;Puppe W;Weigl J;Falcone V
通讯作者：
Falcone V
影响因子：
5.4
作者：
Craig, S;Thu, HM;Aaskov, J
通讯作者：
Aaskov, J
影响因子：
12.7
作者：
Ijpma, FFA;Beekhuis, D;Osterhaus, DME
通讯作者：
Osterhaus, DME
影响因子：
9.4
作者：
Ishiguro, N;Ebihara, T;Kobayashi, K
通讯作者：
Kobayashi, K