UCLA NINDS/NIMH Microarray Center

描述(由申请人提供)： 加州大学洛杉矶分校NINDS/NIMH微阵列中心继续作为NINDS/NIMH微阵列联盟的一个组成部分，将为神经科学界提供广泛的服务、知识和数据资源，以促进基于微阵列的实验的执行和有用的微阵列文献和基因表达信息的存档。通过财团运营的头两年，这三个受资助的网站已经创建了一个由三个中心组成的综合集合，具有互补的专业知识和服务。这些服务受到该联盟的个人用户的好评，对试验的质量给予了很高的评价。我们建议继续适应社区需求，提供知识丰富的指导，并为NINDS/NIMH资助的调查人员提供出色的成本效益服务。通过该联盟提供的服务侧重于大规模基因表达的分析。神经学家使用这一强大技术的努力可能会受挫，原因是1)服务成本高；2)技术可用时受到限制；3)获得专家建议的机会有限；以及4)获得强大而灵活的分析工具的机会有限。加州大学洛杉矶分校的提案与其他两个目前得到资助的中心一起，旨在降低所有这些门槛，并提供现成的访问机会。通过中心之间明智的重叠，最大限度地减少了冗余，并在整个联盟中构建了备份和灵活性。加州大学洛杉矶分校NINDS/NIMH微阵列中心的拟议更新将是一个功能广泛的中心，与其他资助的中心合作，为神经科学界无缝地提供完整的DNA微阵列试剂、服务和数据。该中心将：1)就微阵列实验提供指导和咨询，并促进研究人员对实验程序的记录；2)向神经科学研究人员提供获得DNA微阵列服务的廉价途径；3)为微阵列实验提供定制的阵列合成能力；4)为微阵列数据的分析提供指导、咨询和统计分析支持；5)开发一个注解良好的、交叉引用的、共同标准化的表达数据的中央数据库，侧重于神经科学；以及6)开发和更新加州大学洛杉矶分校的网站与NINDS/NIMH门户网站的整合。

DESCRIPTION (provided by applicant): The continuation of the UCLA NINDS/NIMH Microarray Center as a component of the NINDS/NIMH Microarray Consortium will be a broad based resource of services, knowledge, and data for the neuroscience community to facilitate performance of microarray based experimentation and the archiving of useful microarray documentation and gene expression information. Through the first two years of operation of the Consortium, the three funded sites have created an integrated set of three centers with complementary expertise and services. The services have been well received by the individual users of the consortium, with high marks on quality of the experimentation. We propose to continue to adapt to community needs, provide knowledgeable guidance, and offer an outstanding cost-effective service to NINDS/NIMH funded investigators. The services offered through the Consortium are focused on the analysis of large scale gene expression. Neuroscientists can be frustrated in their efforts to access this powerful technology due to 1) high cost of services; 2) limited access to the technologies as they become available; 3) limited access to expert advice; and 4) limited access to powerful and flexible analytical tools. The UCLA proposal, in conjunction with the other two currently funded centers, serves to lower all of these barriers and provide ready access. Through judicious overlaps between the centers, redundancies are minimized, and backup and flexibility are built into the overall consortium. The proposed renewal of the UCLA NINDS/NIMH Microarray Center will be a broadly functioning center working in collaboration with the other funded centers to seamlessly provide a complete array of DNA microarray reagents, services, and data to the neuroscience community. The Center will: 1) Provide guidance and advice on microarray experimentation and facilitate investigator documentation of experimental procedures; 2) Provide inexpensive access to DNA microarray services to individual neuroscience investigators; 3) Provide custom array synthesis capabilities for microarray experimentation; 4) Provide guidance, advice and statistical analytical support for the analysis of microarray data; 5) Develop a centralized database of well annotated, cross-referenced, co-normalized expression data with a focus on neuroscience; and 6) Develop and update the integration of the UCLA Site with the NINDS/NIMH Portal.