UCLA NINDS/NIMH Microarray Center

描述（由申请人提供）： 继续加州大学洛杉矶分校NINDS/NIMH微阵列中心作为NINDS/NIMH微阵列联盟的一个组成部分，将成为神经科学界广泛的服务，知识和数据资源，以促进基于微阵列的实验和有用的微阵列文件和基因表达信息的存档。通过联合体头两年的运作，三个受资助的地点已经建立了一套由三个中心组成的综合设施，提供互补的专业知识和服务。这些服务得到了联合体个人用户的好评，实验质量得到了高度评价。我们建议继续适应社区需求，提供知识渊博的指导，并为NINDS/NIMH资助的调查人员提供出色的成本效益服务。通过该联盟提供的服务主要集中在大规模基因表达的分析。神经科学家在努力获得这种强大的技术时可能会遇到挫折，原因是：1）服务成本高; 2）获得技术的机会有限; 3）获得专家建议的机会有限; 4）获得强大而灵活的分析工具的机会有限。加州大学洛杉矶分校的建议，与其他两个目前资助的中心，有助于降低所有这些障碍，并提供现成的访问。通过中心之间明智的重叠，冗余被最小化，备份和灵活性被构建到整个联盟中。UCLA NINDS/NIMH微阵列中心的拟议更新将是一个功能广泛的中心，与其他资助中心合作，为神经科学界提供完整的DNA微阵列试剂，服务和数据。该中心将：1）提供关于微阵列实验的指导和建议，并促进研究者对实验程序的记录; 2）为个体神经科学研究者提供廉价的DNA微阵列服务; 3）为微阵列实验提供定制阵列合成能力; 4）为微阵列数据的分析提供指导、建议和统计分析支持; 5）开发一个集中的数据库，集中注释，交叉引用，共同规范化的表达数据，重点是神经科学; 6）开发和更新UCLA网站与NINDS/NIMH门户网站的整合。

DESCRIPTION (provided by applicant): The continuation of the UCLA NINDS/NIMH Microarray Center as a component of the NINDS/NIMH Microarray Consortium will be a broad based resource of services, knowledge, and data for the neuroscience community to facilitate performance of microarray based experimentation and the archiving of useful microarray documentation and gene expression information. Through the first two years of operation of the Consortium, the three funded sites have created an integrated set of three centers with complementary expertise and services. The services have been well received by the individual users of the consortium, with high marks on quality of the experimentation. We propose to continue to adapt to community needs, provide knowledgeable guidance, and offer an outstanding cost-effective service to NINDS/NIMH funded investigators. The services offered through the Consortium are focused on the analysis of large scale gene expression. Neuroscientists can be frustrated in their efforts to access this powerful technology due to 1) high cost of services; 2) limited access to the technologies as they become available; 3) limited access to expert advice; and 4) limited access to powerful and flexible analytical tools. The UCLA proposal, in conjunction with the other two currently funded centers, serves to lower all of these barriers and provide ready access. Through judicious overlaps between the centers, redundancies are minimized, and backup and flexibility are built into the overall consortium. The proposed renewal of the UCLA NINDS/NIMH Microarray Center will be a broadly functioning center working in collaboration with the other funded centers to seamlessly provide a complete array of DNA microarray reagents, services, and data to the neuroscience community. The Center will: 1) Provide guidance and advice on microarray experimentation and facilitate investigator documentation of experimental procedures; 2) Provide inexpensive access to DNA microarray services to individual neuroscience investigators; 3) Provide custom array synthesis capabilities for microarray experimentation; 4) Provide guidance, advice and statistical analytical support for the analysis of microarray data; 5) Develop a centralized database of well annotated, cross-referenced, co-normalized expression data with a focus on neuroscience; and 6) Develop and update the integration of the UCLA Site with the NINDS/NIMH Portal.