Microarray chips for rapid detection of high affinity nucleic acid sequences

描述(申请人提供)：项目摘要/摘要：这个第一阶段的项目旨在展示一种快速发现与蛋白质靶标紧密结合的短序列的方法。我们在第一阶段的计划仅限于通过筛选荧光标记的蛋白质来证明原则，这将有助于分析。在第二阶段，将采用分析的无标签检测方法，努力推广筛查方法。在这些筛查中发现的寡核苷酸可以被设计成传感器，具有极高的灵敏度和选择性，用于诊断和药物发现应用。

DESCRIPTION (provided by applicant): Project Summary/Abstract: This phase I project aims at demonstrating a method to rapidly discover short sequences that bind tightly to protein targets. Our plan in phase I is limited to proof of principle by screening against fluorescently tagged proteins, which will facilitate analysis. In phase II, label-free detection methods of analysis will be pursued in an effort to generalize the screening method. Oligonucleotides discovered in these screens can be engineered into sensors having exquisite sensitivity and selectivity for diagnostics and drug discovery applications.