Quantitative Characterization of UV induced skin damage

描述(由申请人提供)： 这项研究的广泛目标是为理性-智能开发干预和/或修复由于紫外线辐射造成的皮肤损伤的药物提供必要的框架。有待检验的假设是，纳米脂质体包裹的抗氧化剂对皮肤的输送优于脂质体微米级的输送。具体目标包括： 建立紫外线辐射对皮肤损伤的剂量-反应模型。 通过在微米和纳米尺度上传递脂质体中包裹的抗氧化剂来测试预防或修复损伤的效果。 各种维生素的纳米级胶囊脂质体正在制备和测试中。皮肤模型从人类皮肤移植开始，发展到无毛小鼠动物模型，以建立全身水平的反应。对皮肤的损伤将通过观察紫外线损伤的早期标志来表征，例如前胶原合成速度和基质金属蛋白酶的表达。这项拟议的研究将跟踪紫外线在存在和不存在通过微米和纳米递送系统输送的抗氧化剂时所造成的损害。 该项目将通过协同和连接托马斯·杰斐逊医学院、德雷克塞尔大学和候选人之间的专业知识，并调动大学或行业现有的资源，以创建皮肤研究计划，从而实现概述的目标。候选人带来了紫外线降解机制和大分子抗氧化稳定方面的专业知识，这些专业将与导师的专业知识联系在一起；皮肤病学和皮肤生物学(Jouni Uitto和Urich RoDeck)；光生物学(Douglas Brown和Nikiforos Kollias)；封装和传递(Margaret Wheatley)；以及NarioTechnology(Kbiz Pourrezaei)。

DESCRIPTION (provided by applicant): The broad objective of the study is to provide the necessary framework for a rational - smart development of agents to intervene and or repair skin damage due to UV radiation. The hypothesis; to be tested is that nanodelivery of liposome encapsulated antioxidants to the skin is superior to liposome micron size delivery Specific aims include: Develop a dose - response model for the progression of damage induced by UV radiation to skin. Test the efficacy of prevention or repair of damage by delivering antioxidants encapsulated in liposome's at the micro and nanoscale. Encapsulated liposomes of various vitamins down to the nanoscale are being prepared and tested. The skin models start from human skin explants and progress to the hairless mouse animal model to establish response at a systemic level. Damage to the skin will be characterized by looking at early markers of UV damage, such as rate of pro-collagen synthesis, and expression of matrix metalloproteases. The proposed study will track damage caused by UV in the presence and absence of antioxidants delivered through micro and nanodelivery systems. This project will achieve the outlined goals by synergizing and linking expertise between Thomas Jefferson Medical School, Drexel University and the candidate and mobilizing resources currently available at the University or in industry, to create a Skin Research Initiative. The candidate brings expertise in UV degradation mechanisms and antioxidant stabilization of macromolecules that will be linked with the expertise of the mentors; in Dermatology and Skin Biology (Jouni Uitto and Urich Rodeck), in Photobiology (Douglas Brown arid Nikiforos Kollias), in Encapsulation and Delivery (Margaret Wheatley ), and in Nariotechnology (Kambiz Pourrezaei).