Quantitative Characterization of UV induced skin damage

描述（由申请人提供）： 这项研究的主要目的是为合理开发干预和/或修复紫外线辐射引起的皮肤损伤的药物提供必要的框架。假设;要测试的是，脂质体包封的抗氧化剂向皮肤的纳米递送上级脂质体微米尺寸递送。具体目的包括： 建立紫外线辐射对皮肤损伤的剂量反应模型. 测试预防或修复损伤的功效，通过提供封装在脂质体中的抗氧化剂在微米和纳米级。 目前正在制备和测试各种维生素的纳米级封装脂质体。皮肤模型从人皮肤外植体开始，发展到无毛小鼠动物模型，以建立全身水平的反应。对皮肤的损伤将通过观察UV损伤的早期标志物来表征，例如前胶原合成速率和基质金属蛋白酶的表达。拟议的研究将跟踪在存在和不存在通过微米和纳米递送系统递送的抗氧化剂的情况下由紫外线引起的损伤。 该项目将通过协同和联系托马斯杰斐逊医学院，德雷克塞尔大学和候选人之间的专业知识，并调动目前在大学或行业可用的资源，以实现概述的目标，创建一个皮肤研究计划。候选人带来了紫外线降解机制和大分子抗氧化剂稳定性方面的专业知识，这些知识将与导师的专业知识相联系;皮肤病学和皮肤生物学（Jouni Uitto和Urich Rodeck），光生物学（道格拉斯布朗和Nikiforos Kollias），封装和交付（Margaret惠特利）以及Nariotechnology（Kambiz Pourrezaei）。

DESCRIPTION (provided by applicant): The broad objective of the study is to provide the necessary framework for a rational - smart development of agents to intervene and or repair skin damage due to UV radiation. The hypothesis; to be tested is that nanodelivery of liposome encapsulated antioxidants to the skin is superior to liposome micron size delivery Specific aims include: Develop a dose - response model for the progression of damage induced by UV radiation to skin. Test the efficacy of prevention or repair of damage by delivering antioxidants encapsulated in liposome's at the micro and nanoscale. Encapsulated liposomes of various vitamins down to the nanoscale are being prepared and tested. The skin models start from human skin explants and progress to the hairless mouse animal model to establish response at a systemic level. Damage to the skin will be characterized by looking at early markers of UV damage, such as rate of pro-collagen synthesis, and expression of matrix metalloproteases. The proposed study will track damage caused by UV in the presence and absence of antioxidants delivered through micro and nanodelivery systems. This project will achieve the outlined goals by synergizing and linking expertise between Thomas Jefferson Medical School, Drexel University and the candidate and mobilizing resources currently available at the University or in industry, to create a Skin Research Initiative. The candidate brings expertise in UV degradation mechanisms and antioxidant stabilization of macromolecules that will be linked with the expertise of the mentors; in Dermatology and Skin Biology (Jouni Uitto and Urich Rodeck), in Photobiology (Douglas Brown arid Nikiforos Kollias), in Encapsulation and Delivery (Margaret Wheatley ), and in Nariotechnology (Kambiz Pourrezaei).