Mosquito-malaria interactions in the hemocoel

描述（由申请人提供）：蚊子传播致病病原体。疟原虫是疟疾的病原体，每年导致数百万人死亡。为了将疟原虫自然传播到脊椎动物宿主，寄生虫在蚊子载体中经历了一系列强制性的发育和繁殖过程。目前应用的兴趣是蚊子血中疟原虫孢子期的生物学。初步实验已经在冈比亚按蚊中发现了一个与埃及伊蚊唾液腺孢子子受体同源的蛋白家族。在这里，我们将描述这些An的时空表达。冈比亚菌蛋白，并确定其参与唾液腺的孢子入侵。此外，唾液腺的侵袭是一个非常低效的过程。许多孢子子无法侵入血液，并迅速死亡。在这里，我们还将尝试描述蚊子杀死孢子虫的内源性机制。了解寄生虫在病媒内完成其生命周期的机制，以及病媒杀死寄生虫的机制，可能会带来新的传播控制策略。

DESCRIPTION (provided by applicant): Mosquitoes transmit disease-causing pathogens. Plasmodium parasites alone, the causative agents of malaria, kill millions of people each year. For natural transmission of Plasmodium to the vertebrate host, the parasite undergoes a series of obligatory developmental and propagative processes in a mosquito vector. Of interest in the current application is the biology of the sporogonic stages of Plasmodium in the mosquito hemocoel. Preliminary experiments have identified a family of proteins in the mosquito Anopheles gambiae with homology to an Aedes aegypti putative salivary gland sporozite receptor. Here, we will characterize the temporal and spatial expression of these An. gambiae proteins, and determine their involvement in salivary gland invasion by sporozoites. Furthermore, salivary gland invasion is a very inefficient process. Many sporozoites are unable to invade and rapidly die in the hemocoel. Here, we will also attempt to characterize the endogenous mechanisms used by mosquitoes to kill sporozoites. Understanding the mechanisms used by parasites to complete their life cycle inside the vector, and the mechanisms used by the vector to kill parasites, may lead to novel transmission control strategies.