HIV Vaccines via 2G12 Carbohydrate Epitope Mimicry

描述（由申请人提供）：提出了一种HIV碳水化合物表位疫苗的合成。疫苗的设计是基于人类抗体2G12与HIV蛋白gp120表面上发现的寡糖结合相互作用的最新结晶学信息。本研究的结果表明，2G12与几个聚糖簇之间的多价相互作用的协同性使得2G12-gp120的强结合成为可能。在本实验室合成的疫苗将试图模拟两种或两种以上聚糖的空间呈现，以形成类似的簇。在初步设计完成后，疫苗将与生物学家合作进行测试，看它是否能刺激2g12样抗体的产生，这种抗体能够中和大量的HIV-1分离株。根据这些研究的结果，将重新设计、重新合成和重新测试疫苗。这个过程的许多循环将有望教会我们很多关于疫苗、艾滋病毒和有机合成的知识。

DESCRIPTION (provided by applicant): The synthesis of an HIV carbohydrate epitope vaccine is proposed. The vaccine design is based upon recent crystallographic information about the binding interactions of human antibody 2G12 with the oligosaccharides found on the surface of the HIV protein gp120. The findings in this study suggest that the strong 2G12-gp120 binding is made possible by the cooperativity of a multivalent interaction between 2G12 and a cluster of several glycans. The vaccine synthesized in this laboratory will seek to mimic this spatial presentation of two or more glycans to form a similar cluster. After the initial design has been synthesized, the vaccine will then be tested in collaboration with biologists, to see whether it stimulates the production of 2G12-like antibodies, capable of neutralizing a broad range of HIV-1 isolates. Based on the results of these studies, the vaccine will be redesigned, resynthesized and retested. Many cycles of this process will hopefully teach us a great deal about vaccines, HIV, and organic synthesis.