Technologies for Directed Evolution of Glycoaptamers
糖适体定向进化技术
基本信息
- 批准号:10721663
- 负责人:
- 金额:$ 42.12万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2023
- 资助国家:美国
- 起止时间:2023-09-01 至 2027-08-31
- 项目状态:未结题
- 来源:
- 关键词:AccelerationAcidsAffinityAllergensAtherosclerosisAutoimmunityAvidityBar CodesBindingBinding SitesBiologicalBiological ProcessC-Type LectinsCD209 geneCarbohydratesCategoriesCell AdhesionChemicalsCommunicable DiseasesComplexDNADefense MechanismsDiabetes MellitusDiagnosticDirected Molecular EvolutionDiseaseElementsEvolutionExhibitsEyeFibrosisGalactoseGalactose Binding LectinGalectin 1GenerationsGeometryGlycobiologyGoalsHIV AntibodiesHIV vaccineHandHeartImmunityImmunosuppressionIndividualInternetInterruptionLectinLibrariesLigand BindingLigandsLinkLiverLungMacrophageMalignant NeoplasmsMannoseMedicineMethodsModificationNamesNucleic AcidsPharmaceutical ChemistryPharmaceutical PreparationsPolymersPolysaccharidesProcessPropertyProtein-Carbohydrate InteractionProteinsRNAResearchResistanceRheumatoid ArthritisRoleSerumSialic AcidsSignal TransductionSpecificityTechnologyTestingValidationVariantVertebral columnViralVirus DiseasesWorkangiogenesisaptamercarbohydrate binding proteincarbohydrate receptorchelationdesensitizationdesignflexibilityglycosylationimmunoregulationin vivoinhibitorinterestlangerinmembernon-drugnucleasepathogenprotein functionrapid techniqueresponsescaffoldsialic acid binding Ig-like lectinsmall moleculesugartherapeutic developmenttool
项目摘要
Project Summary/Abstract
The goal of this Focused Technology R01 project is to develop technologies for rapid discovery
of inhibitors of carbohydrate-binding proteins (CBPs). Carbohydrate-protein interactions are
critical in numerous processes including host-pathogen recognition, cell adhesion and cell
signaling, cancer angiogenesis, immune suppression, heart/liver/lung/eye fibrosis,
atherosclerosis, diabetes, and rheumatoid arthritis, to name a few examples. However, selective
disruption of specific CBP interactions is challenging in vivo. CBPs generally bind weakly to
individual glycans, with higher affinity (avidity) or specificity coming from multivalent interactions
to clustered glycans or interactions with nearby non-carbohydrate elements. A given CBP also
usually binds to various glycans. To untangle the complex web of CBP functions, it is important
to have a way to rapidly develop a specific and potent inhibitor of a CBP that can be used in the
relevant in vivo context. To this end, we propose two Aims: 1) to develop multivalent glyco-F-RNA
aptamers, in which a nuclease-resistant, F-RNA backbone is evolved to present simple glycans
in a manner that is selectively recognized by a CBP of interest; 2) to develop monovalent glyco-
F-RNA aptamers, in which a simple glycan provides a binding “foothold” for the CBP of interest,
which the evolved F-RNA component confers additional binding affinity and specificity through
interactions auxiliary to the glycan-binding pocket.
项目总结/文摘
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Isaac Jonathan Krauss其他文献
Isaac Jonathan Krauss的其他文献
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{{ truncateString('Isaac Jonathan Krauss', 18)}}的其他基金
Mannosidase-stabilized glycan immunogens for elicitation of high mannose patch antibodies
甘露糖苷酶稳定的聚糖免疫原,用于引发高甘露糖贴片抗体
- 批准号:
10619737 - 财政年份:2023
- 资助金额:
$ 42.12万 - 项目类别:
Design of Immunogens to Elicit PGT122 like Antibodies
设计免疫原以引发 PGT122 样抗体
- 批准号:
8776122 - 财政年份:2014
- 资助金额:
$ 42.12万 - 项目类别:
Design of Immunogens to Elicit PGT122 like Antibodies
设计免疫原以引发 PGT122 样抗体
- 批准号:
9506647 - 财政年份:2014
- 资助金额:
$ 42.12万 - 项目类别:
Design of Immunogens to Elicit PGT122 like Antibodies
设计免疫原以引发 PGT122 样抗体
- 批准号:
9297203 - 财政年份:2014
- 资助金额:
$ 42.12万 - 项目类别:
Glycopeptide evolution targeting antibodies of the PGT128 lineage.
糖肽进化靶向 PGT128 谱系的抗体。
- 批准号:
9392707 - 财政年份:2010
- 资助金额:
$ 42.12万 - 项目类别:
Glycoantigen evolution targeting antibodies of the PGT 128 lineage
糖抗原进化靶向 PGT 128 谱系抗体
- 批准号:
10062844 - 财政年份:2010
- 资助金额:
$ 42.12万 - 项目类别:
Rational and Combinatorial Design of Immunogens to Elicit 2G12-like Antibodies
合理组合设计免疫原以引发 2G12 样抗体
- 批准号:
7988539 - 财政年份:2010
- 资助金额:
$ 42.12万 - 项目类别:
Glycoantigen evolution targeting antibodies of the PGT 128 lineage
糖抗原进化靶向 PGT 128 谱系抗体
- 批准号:
10312026 - 财政年份:2010
- 资助金额:
$ 42.12万 - 项目类别:
Rational and Combinatorial Design of Immunogens to Elicit 2G12-like Antibodies
合理组合设计免疫原以引发 2G12 样抗体
- 批准号:
8287717 - 财政年份:2010
- 资助金额:
$ 42.12万 - 项目类别:
Rational and Combinatorial Design of Immunogens to Elicit 2G12-like Antibodies
合理组合设计免疫原以引发 2G12 样抗体
- 批准号:
8478035 - 财政年份:2010
- 资助金额:
$ 42.12万 - 项目类别:
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