600 MHz NMR Spectrometer for Shared Use

描述(由申请人提供)：约翰·霍普金斯大学霍姆伍德校区需要一台高场核磁共振光谱仪，工作在600 MHz的1H共振频率，并为生物分子应用提供完整的设备，以满足广泛的生物物理和生化研究人员的需求。所要求的仪器将设在一个新建成的核磁共振中心，该中心的建造符合该大学的长期目标，即为生物分子结构和动力学的基础研究提供最先进的能力。这项仪器将使生物学、生物物理学和化学系的七个学院的研究计划大大受益，其中六个是由美国国立卫生研究院资助的。描述了一系列具有生物学、生物物理和化学意义的项目：(1)参与线粒体分裂的蛋白质的结构和动力学研究。(2)大分子结构和动力学的核磁共振研究及其与生物功能的关系。(3)HIV-1蛋白酶的氨基酸多态和突变导致耐药的长期效应。(4)膜蛋白的结构和能量原理。(5)蛋白质结构与能量的关系。(6)AraC DNA结合区的结构测定及其同源DNA存在和不存在时的动态变化。(7)DNA存在和不存在时DNA结合蛋白主链和侧链的热力学分析。

DESCRIPTION (provided by applicant): A high-field NMR spectrometer, operating at a 1H resonance frequency of 600 MHz and fully equipped for biomolecular applications, is requested to meet the needs of a broad group of biophysical and biochemical investigators at the Homewood campus at Johns Hopkins University. The requested instrumentation will be sited in a newly completed NMR Center, constructed in accordance with the University's long term goal of providing state-of-the-art capabilities for fundamental investigations of biomolecular structure and dynamics. This instrumentation will significantly benefit the research programs of seven faculties from the Departments of Biology, Biophysics, and Chemistry, of which six are NIH-funded. A range of projects of biological, biophysical, and chemical significance are described: (1) Structure and dynamics studies of proteins involved in mitochondrial division. (2) NMR studies of macromolecular structure and dynamics and its relationship to biological function. (3) Long range effects of amino acid polymorphisms and mutations causing drug-resistant in the HIV-1 protease. (4) Structural and energetic principles of membrane proteins. (5) Correlation between structure and energy in proteins. (6) Structure determination of the DNA-binding domain of AraC and dynamics in the presence and absence of its cognate DNA. (7) Thermodynamic analysis of backbone and side chain dynamics of DNA binding proteins in the presence and absence of DNA.