PharmaScan 70/16 In-Vivo Spectroscopy/Imaging System

描述(由申请人提供)： 随着最近的遗传学和分子水平的进步，人类疾病的小动物(大鼠/小鼠)模型已经成为研究疾病潜在机制的越来越重要的资源。许多传统的研究方法需要牺牲动物来进行体外组织和分子分析。这阻止了研究人员在体内观察正在研究的过程的自然或受干扰的进化。此外，小动物模型正成为研究新型可植入微型设备和生物材料修复、再生或取代生命系统的能力的越来越重要的试验台。小动物规模的成像提供了一个机会，可以非侵入性地废除对同一动物体内生物过程的研究，并有效地测试疾病的治疗方法。 生物成像的一种方法是使用核磁共振。亚利桑那州立大学缺乏在小动物或大组织样本中进行活体成像和光谱分析的能力。差距进一步扩大，因为整个都会凤凰谷地区缺乏这样的能力，那里有几个优秀的临床和研究医疗设施，距离最近的设施(120英里)不利于对大量小动物进行纵向慢性研究，以支持凤凰谷的研究需求。我们请求布鲁克生物系统公司提供PharmaScan 70116 7.0Tesla，90厘米净径系统的支持。这是一个多用途的研究扫描仪，用于高分辨率、快速、核磁共振2D和3D图像重建以及活体光谱分析。几名研究人员将在他们的研究中使用这一系统，从而大大受益。应用范围从评估肿瘤的化疗效果，到在含有AD基因的双转基因小鼠中开发、测试和实施阿尔茨海默病(AD)的非侵入性脑成像指标，到评估脊髓损伤或中风后中枢神经系统的可塑性。

DESCRIPTION (provided by applicant): With recent genetic and molecular advances, small animal (rat/mice) models of human disease have become increasingly important resources for the investigation of the underlying mechanisms of disease. Many traditional investigational approaches require sacrificing the animals for ex vivo tissue and molecular analysis. This prevents the researchers from observing in vivo the natural or perturbed evolution of the processes under study. Additionally, small animal models are becoming increasingly important test beds to investigate the ability of novel implantable miniaturized devices and biomaterials to repair, regenerate or replace the living system. Imaging on the scale of small animals offers an opportunity to noninvasively repeal investigations of biological processes in vivo in the same animal and efficiently test treatments for disease. One approach for bioimaging is to use nuclear magnetic resonance. The ability to perform in vivo imaging and spectroscopy in small animals or large tissue samples is absent at Arizona State University. The gap is further enhanced because of a lack of such a capability in the entire Metropolitan Phoenix Valley area that is home to several excellent clinical and research medical facilities The distance to the closest facility (120 miles) is not conducive to conducting longitudinal chronic studies on large numbers of small animals to support the needs of research in the Phoenix Valley. We request support for a PharmaScan 70116 7.0 Tesla, 90 cm clear bore system from Bruker-Biospin. This is a multipurpose research scanner for high resolution, fast speed, Nuclear Magnetic Resonance 2D and 3D image reconstruction and in vivo spectroscopy. Several investigators would significantly benefit from utilizing this system in their research. The applications would range from assessing effects of chemotherapy for tumors, to developing, testing, and implementing noninvasive, brain-imaging indicators of Alzheimer's Disease (AD) in double transgenic mice containing AD genes, to assessment of CNS neuroplasticity after spinal cord injury or stroke.