Integrated Proteom Works System

描述（由申请人提供）：本提案的目的是寻求购买半机器人Proteome Works系统的资金。这种集成的仪器和软件包将大大提高质谱实验室与克利夫兰临床基金会（CCF）的Lerner研究所的能力。所要求的集成蛋白点切割器，蛋白质蛋白水解消化/处理/MALDI点样站和软件将用于促进和推进该地区机构（CCF和凯斯西储大学）的研究计划。主要用户都有许多NIH资助的研究经费，其中有组成项目，可以最好地使用2-D凝胶分析，然后进行质谱分析。所有主要用户的项目都需要大量的样品制备和/或2-D凝胶分析和比较。主要用户项目范围广泛，包括：1）鉴定正常和病变组织中差异表达的蛋白质; 2）定量复杂生物复合物中结构确定的组分; 3）表征共价蛋白质修饰; 4）研究细胞分化标志物和改变的基因表达。Proteome Works系统提供了满足这些不同需求的多功能性、分辨率和功能。此外，所要求的仪器系统将用于满足偶尔使用者的需要。这项服务将在我们现有的分子生物技术核心内进行，该核心通过进行蛋白质测序、肽合成和相关服务为研究人员提供支持。由于人类基因组计划，质谱核心的使用量大大增加。借助集成的Proteome Works系统，质谱核心可以处理更多样品并提高其机器时间效率。重要的是，Proteome Works系统将允许用户数字化捕获2-D凝胶图像，比较多个实验的结果，并整合基因表达信息，并将其直接与蛋白质身份和表达水平相关联。事实上，无法做到这一点大大阻碍了生物医学研究的进展。收购蛋白质组工程系统将允许充分利用独特的能力和有用的质谱核心，基因表达实验室和核心在CCF和CWRU这里。CCF已投入大量资源用于CCF质谱实验室的发展，包括监督其运营的人员，以及确保其长期运营和维护的财政计划。总之，收购所要求的集成Proteome Works系统将对该地区多个机构（包括CCF和CWRU）的生物医学研究计划的进展和未来发展产生重大影响。

DESCRIPTION (provided by applicant): The purpose of this proposal is to seek funds for the purchase of a semi-robotic Proteome Works system. This integrated instruments and software package will greatly enhance the Laboratory of Mass Spectrometry with the Lerner Research Institute of the Cleveland Clinic Foundation (CCF) capabilities. The requested integrated protein spot cutter, protein proteolytic digestion/processing/MALDI-spotting station and software will be used to facilitate and advance research programs at institutions within the area (CCF and Case Western Reserve University). Major Users all have numerous NIH-funded research grants, which have component projects that can best be conducted using 2-D gel analysis followed by mass spectrometry. All the Major User's projects require substantial numbers of sample preparations and/or 2-D gel analysis and comparison. The Major Users projects are broad and include: 1) identification of differentially expressed proteins in normal and diseased tissue; 2) quantification of structurally defined components in complex biological complexes; 3) characterization of covalent protein modifications; and 4) investigations of cellular differentiation markers and altered gene expression. The proteome Works system provides the versatility, resolution and capabilities to meet these diverse demands. In addition, the requested instrument system will be used to meet the needs of the occasional user. This service will be conducted within our existing Molecular Biotechnology Core, which provides support to investigators by performing protein sequencing, peptide synthesis, and related services. Due to the human genome project, the Mass Spectrometry Core has experienced greatly increased usage. With the integrated Proteome Works system, the mass Spectrometry Core can process more samples and increase its machine-time efficiency. Importantly, the Proteome Works system will allow users to digitally capture 2-D gel images, compare results of multiple experiments and integrate in gene expression information and relate it directly back to protein identities and expression levels. In fact, the inability to do this has significantly hindered the pace of progress in biomedical research. Acquisition of the Proteome Works system will allow full utilization of the unique capabilities and usefulness of the Mass Spectrometry Core, gene expression laboratories and Cores here at CCF and CWRU. CCF has committed substantial resources to the development of the CCF Laboratory of Mass Spectrometry, including personnel to oversee its operation, and a fiscal plan to insure its operation and maintenance for the long-term. In sum, acquisition of the requested integrated Proteome Works system will have a significant impact on the progress and future development of biomedical research programs at multiple institutions in the region including CCF and CWRU.