MALDI TOF TOF mass spectrometer

描述（由申请人提供）：质谱法在生物分子研究中的应用已经彻底改变了生物医学研究的许多方面。位于UMDNJ-Robert Wood Johnson医学院和罗格斯大学，新泽西州立大学（Piscataway/New Brunswick校区）的30个nih支持的研究小组正在申请应用生物系统4700蛋白质组学分析仪MALDI TOF TOF质谱仪，以取代和升级我们现有的共享MALDI-TOF质谱仪资源。用户组成员建议使用该仪器进行生物医学相关的蛋白质组学研究、蛋白质鉴定、重组蛋白、多肽和生物偶联物的质量控制、翻译后和化学修饰的表征、交联分析、氘交换以及物理和结构生物学研究。用户组的大多数成员目前将质谱法作为其研究的一个组成部分，并要求继续使用MALDI质谱仪，并且将从所要求的仪器的能力增加中受益匪浅。这将导致生产力的显著提高。更重要的是，这种资源将使研究人员能够在新的方向上扩展他们的研究，并开始以前无法进行的新项目。

DESCRIPTION (provided by applicant): The application of mass spectrometry to the study of biological molecules has revolutionized many aspects of biomedical research. A group of thirty NIH-supported research groups located at UMDNJ-Robert Wood Johnson Medical School and Rutgers, the State University of New Jersey (Piscataway/New Brunswick Campus) are requesting an Applied Biosystems 4700 Proteomics Analyzer MALDI TOF TOF mass spectrometer to replace and upgrade our existing shared MALDI-TOF mass spectrometry resource. The user group members propose to use this instrument to perform biomedically relevant proteomics studies, protein identification, quality control of recombinant proteins, peptides, and bioconjugates, characterization of post-translational and chemical modifications, cross-linking analysis, deuterium exchange, as well as physical and structural biology studies. Most members of the User Group currently employ mass spectrometry as an integral part of their research and require continued access to a MALDI mass spectrometer, and would benefit immensely from the increased capabilities of the requested instrument. This would result in significant increases in productivity. More importantly, this resource will allow the investigators to expand their research in new directions, and to begin new projects that could not have previously been undertaken.