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A Mass Spectrometry System for Quantitative Proteomics

用于定量蛋白质组学的质谱系统

基本信息

批准号：
8640415
负责人：
PETER LOBEL
金额：
$ 56.13万
依托单位：
RBHS-ROBERT WOOD JOHNSON MEDICAL SCHOOL
依托单位国家：
美国
项目类别：
财政年份：
2014
资助国家：
美国
起止时间：
2014-07-01 至 2015-06-30
项目状态：
已结题

项目摘要

DESCRIPTION (provided by applicant): This is an application requesting funds to purchase a Q Exactive mass spectrometer and liquid chromatography system that will be used for quantitative proteomics research. The instrument will be integrated into the Robert Wood Johnson Medical School/Rutgers University Biological Mass Spectrometry Facility. Current instrumentation in the Facility is used at maximal capacity and we require additional capabilities to conduct quantitative proteomics experiments for thirteen NIH-funded investigators. Our user group will apply quantitative mass spectrometry to most efficiently address a wide range of important biomedical research areas including neurological disease, bacterial and viral infection, cancer, anaphylaxis, diabetes, digestive disease, and sleep disorders. Specific experiments include quantification of changes in levels and post-translational modifications of clock proteins throughout the circadian cycle, a systems biology approach to understand the lysosome and its role in human neurodegenerative disease, persistence of epitopes on protein food allergens under conditions that simulate gastric digestion, dynamics of post- translational modification of tumor suppressors during oncogenesis and analysis of pathogen-host interactions at different stages in their life cycle for hepatitis C virus, retroviruses, and chlamydia.. The requested instrument will therefore represent a significant asset to our research community and will accelerate the pace of basic and applied NIH-funded health-research at Rutgers University and the Robert Wood Johnson Medical School of UMDNJ.

描述（由申请人提供）：这是一份申请，要求提供资金购买Q Exactive质谱仪和液相色谱系统，用于定量蛋白质组学研究。该仪器将被集成到罗伯特伍德约翰逊医学院/罗格斯大学生物质谱设施。目前的仪器在设施中使用的最大容量，我们需要额外的能力进行定量蛋白质组学实验的13个NIH资助的研究人员。我们的用户组将应用定量质谱法最有效地解决广泛的重要生物医学研究领域，包括神经系统疾病，细菌和病毒感染，癌症，过敏反应，糖尿病，消化系统疾病和睡眠障碍。具体的实验包括在整个昼夜节律周期中量化时钟蛋白的水平和翻译后修饰的变化，理解溶酶体及其在人类神经退行性疾病中的作用的系统生物学方法，在模拟胃消化的条件下蛋白质食物过敏原上的表位的持久性，肿瘤发生过程中肿瘤抑制因子翻译后修饰的动力学和丙型肝炎病毒生命周期不同阶段病原体-宿主相互作用的分析，逆转录病毒和衣原体。因此，所要求的仪器将是我们研究界的一项重要资产，并将加快罗格斯大学和UMDNJ的罗伯特伍德约翰逊医学院的基础和应用NIH资助的健康研究的步伐。

项目成果

期刊论文数量（10）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

A multipronged approach unravels unprecedented protein-protein interactions in the human 2-oxoglutarate dehydrogenase multienzyme complex.

多管齐下的方法揭示了人类 2-酮戊二酸脱氢酶多酶复合物中前所未有的蛋白质-蛋白质相互作用。

DOI：
10.1074/jbc.ra118.005432
发表时间：
2018
期刊：
The Journal of biological chemistry
影响因子：
0
作者：
Zhou,Jieyu;Yang,Luying;Ozohanics,Oliver;Zhang,Xu;Wang,Junjie;Ambrus,Attila;Arjunan,Palaniappa;Brukh,Roman;Nemeria,NataliaS;Furey,William;Jordan,Frank
通讯作者：
Jordan,Frank

Elemental sulfur reduction in the deep-sea vent thermophile, Thermovibrio ammonificans.

深海喷口嗜热菌、氨化热弧菌中元素硫的还原。