Multi-omics investigation of blood cell development

输血、骨髓干细胞移植和免疫疗法是医疗和癌症治疗的基石。多能干细胞可以代表这些治疗相关细胞产品的无限且急需的来源。然而，我们生产这些细胞的能力受到我们对血液发育缺乏深入了解的限制。此外，了解胚胎血液发育也将为婴儿白血病的细胞起源和发展机制提供宝贵的线索。这个博士项目的主要目的是研究血液系统的发展，使用最先进的体内/体外小鼠模型，流式细胞术，造血测定，单细胞组学方法（单细胞RNA和ATAC-seq，空间转录组学）结合大规模高维单细胞多模式组学的先进生物信息学整合。

Blood transfusions, bone marrow stem cells transplantations and immunotherapies are cornerstones of medical and cancer treatments. Pluripotent stem cells could represent an unlimited, and much needed, source for these therapeutically relevant cell products. However, our ability to produce these cells is limited by our lack of deep understanding of blood development. In addition, understanding embryonic blood development would also provide invaluable clues on the cellular origin and mechanisms of development of infant leukaemia. The main aim of this PhD project is to investigate the development of the blood system using state of the art in vivo/in vitro mouse models, flow cytometry, haematopoietic assays, single-cell omics approaches (single-cell RNA and ATAC-seq, spatial transcriptomic) combined with advanced bioinformatic integration of large scale high dimensional single-cell multi-modal omics.