Capillary Electrophoresis Profiling of Tears in Dry Eye

描述：(申请人摘要)我们建议研究和开发一种 一种廉价可靠的脂肪和蛋白质组成测定方法 可以在基础科学实验室和临床上使用的泪水 在诊断实验室的环境中。我们的方法不仅会生成 更准确地诊断干眼症所需的详细信息， 也为更具体、更有效的发展提供了依据 针对个人的类别(可能还包括直接针对个人的治疗 干眼症的特征之一就是眼部缺陷。多过 仅在美国就有1200万人被临床诊断 伴有某种形式的角膜炎、干燥症或干眼症。尽管他们的症状是 同样，根本原因往往是未知的，也没有实用的方法 用于评估这类患者泪液成分的方法是存在的。到目前为止， 泪液成分的分析一直受到微小样本量的阻碍 是否可用，以及在技术上无法识别和测量 这种复杂的流体在纳米分子或更小的浓度范围内。毛细管 电泳-电喷雾电离质谱仪(CE-ESI/MS)是一种 灵敏、相对便宜的方法，能够分析样品2pl或 尺寸要小一些。使用胶束电动毛细管色谱技术 结合质谱学检测，我们计划设计和开发1)一种 泪液中脂类的新标准分离方法和2)新方法 泪液中蛋白质的标准分离方法。到时候我们会的 研究使用这些方法来开发标准的脂肪和蛋白质 正常泪液的成分特征，并确定特定成分的差异 两个不同的干眼症模特的眼泪。这方面的发展 提供有关干燥症的特定信息的技术 眼睛可以缓解和提高数百万人的生活质量 目前没有可靠的长期治疗方法的患者。

DESCRIPTION: (Applicant's Abstract) We propose to investigate and develop an inexpensive and reliable method to determine the lipid and protein composition of tears that could be used both in the basic science laboratory and clinically in a diagnostic laboratory setting. Our approach would not only generate the detailed information needed for more precise diagnosis of dry eye conditions, but also provide the basis for development of more specific and effective categories for (and possibly therapies targeted directly to) the individual deficiencies that ' characterize the spectrum of dry eye disorders. More than 12 million people in the United States alone have been clinically diagnosed with some form of keratitis sicca or dry eyes. Although their symptoms are similar, the underlying causes are often unknown and no practical methodology for evaluating the composition of tears from such patients exists. To date, analysis of tear composition has been hampered by the minute sample sizes available and the technical inability to identify and measure the components of such a complex fluid at concentration scales of nanomoles or less. Capillary electrophoresis-electrospray ionization mass spectrometry (CE-ESI/MS) is a sensitive, relatively inexpensive method capable of analyzing samples 2 pl or less in size. Using micellar electrokinetic capillary chromatography techniques coupled with mass spectrometry detection, we plan to design and develop 1) a novel standard separation method for the lipids within tears and 2) a novel standard separation method for the proteins within tears. We will then investigate the use of these methods to develop standard lipid and protein component profiles of normal tears and identify specific component differences in the tears from two different dry eye models. The development of this technology to provide specific information on the complex disorder know as dry eye could provide relief to and enhance the quality of life of millions of patients for whom no reliable long-lasting therapy is currently available.