Microscopy and Shared Equipment

NK细胞的功能取决于细胞表面表达的抑制性和激活性受体之间的平衡，以及它们在NK细胞-靶细胞突触中的相对组织方式。因此，该计划的一个主要目标是了解人类NK细胞功能的细胞生物学。这四个单位提出的形态学研究包括NK受体和配体在细胞内区室的定位以及NK细胞-靶细胞突触的细胞表面分布检查。此外，将定义影响受体表达的膜交通途径。所有这些细胞生物学研究都涉及使用本中心提供的反褶积显微镜设备。为了在受控环境下了解单个NK受体的细胞生物学，许多提出的研究将利用通过将受体引入NKL细胞系或将配体引入NKL细胞系而产生的转染剂

NK cell function depends on the balance between inhibitory and activating receptors expressed on the cell surface and how they are organized relative to each other in the NK cell-target cell synapse. For this reason a major goal of the program is to understand the cell biology of human NK cell function. Morphological studies proposed by all four units include localization of NK receptors and ligands in intracellular compartments and examination of their cell surface distribution at the NK cell-target cell synapse. In addition, membrane traffic pathways affecting receptor expression will be defined. All of these cell biological studies involve use of a deconvolution microscope facility to be provided by this Core. To understand the cell biology of individual NK receptors in a controlled context, many of the studies proposed will make use of transfectants produced by introduction of receptors into the NKL cell line or ligands into the 721.221 cell line. In order to produce such transfectants, this Core provides a shared AMAXA Nucleofector instrument. The Core also supports a shared Staff Research Associate (50% effort) who will be supervised by the Director, provide technical assistance for Core utilization and provide training in use of the Core facilities to personnel of the program. The specific aims of this Microscopy and Shared Equipment Core are to 1) support and maintain the deconvolution microscopy facility for the program and 2) purchase, support and maintain an AMAXA Nucleofector.