Core--Chimerism Analysis and Canine Histocompatibility

这一核心是为了满足本计划项目赠款上所有四个研究项目的需要而设计的。核心人员包括核心主任、一名全职技术人员和一名兼职研究技术人员。I核有两个组件。一个是在非清髓性调节和造血细胞移植后提供狗(项目1和2)和人类患者(项目3和4)的嵌合体分析。这些分析将使用基于聚合酶链式反应(PCR)的微卫星标记多态分析进行。这些研究将用于监测所有四个国家的供体植入程度。 研究项目。第二个目标是为项目1和项目2提供犬组织相容性分型，目的是选择匹配的犬白细胞抗原(DLA)供受者对进行移植。

This core is designed to meet the needs of all four research projects on this Program Project Grant. The core personnel consist of the Core Director, one full-time Technician and one half-time Research Technician. The I core has two components. One will be to provide chimerism analyses in dogs (Projects 1 and 2) and human patients (Projects 3 and 4) after nonmyeloablative conditioning and hematopoietic cell transplantation. These analyses will be performed using polymerase chain reaction (PCR)-based microsatellite marker polymorphism assays. These studies will serve to monitor the degree of donor engraftment in all four research projects. The second goal will be to provide canine histocompatibility typing for Projects 1 and 2 with the aim of selecting dog leukocyte antigen (DLA) identical littermate donor-recipient pairs for transplantation.