POROUS POLYMER RODS AS CHROMATOGRAPHIC OR REACTIVE MEDIA

描述（由申请人提供）：本续提案旨在设计，制备和测试全新的毛细管和片状整体柱，为生物活性分子的研究，分离，分离，检测和鉴定提供无与伦比的潜力，并能够充分利用生命科学研究的丰富潜力。这些新柱将通过一个简单的自由基聚合过程制备。优选的实施方案将包括制备具有针对所需应用优化的多孔特性的通用大孔整体聚合物。然后，孔隙表面将提供各种高密度功能，包括疏水性，亲水性，可电离性和使用UV引发的光接枝反应性部分。这种高度通用的方法也将用于创建具有前所未有的顺序和分层表面化学的单体。聚合和接枝这两个制备步骤都是独立、简单、无浪费、劳动强度低的，可以快速开发出各种定制的单片材料。整体毛细管柱将专门设计用于微高效液相色谱中的高通量和/或多维分离，以及毛细管和薄层电色谱。使用微高效液相色谱法，分离困难的蛋白质混合物将探索使用柱精心设计的选择性。全新的CEC分离模式将由接枝化学的进展而开发，并用于快速有效地分离蛋白质和肽。利用大孔聚合物薄片的薄层色谱和相关方法的新方法将集中在生物大分子的更大规模和二维分离上，并将包括压力和电驱动流。该提案还针对可用于处理生物样品的增强单片工具阵列的开发。利用顺序光接枝的优点，将在电喷雾发射器的尖端制备单片器件，该器件将集成样品收集，酶解蛋白质和肽分离。该装置与质谱仪的直接耦合将简化和加快蛋白质组学研究。总体而言，我们的目标是扩展单片材料在新领域的应用，增强其性能和意想不到的能力，并展示其在众多领域的巨大潜力。

DESCRIPTION (provided by applicant): This continuation proposal aims at the design, preparation, and testing of completely new monolithic columns in capillary and sheet formats that will provide unmatched potential for the study, isolation, separation, detection, and identification of biologically active molecules and enable to fully exploit the rich potential of studies in life sciences. These new columns will be prepared via a simple free radical polymerization process. The preferred implementation will include preparation of a generic macroporous monolithic polymer with porous properties optimized for the desired application. The pore surface will then be provided with a wide variety of high-density functionalities including hydrophobic, hydrophilic, ionizable, and reactive moieties using UV initiated photografting. This highly versatile approach will also be used to create monoliths with unprecedented sequential and layered surface chemistries. Both preparation steps - polymerization and grafting - are independent, simple, waste-free, as well as less labor intensive, and enable the rapid development of a wide variety of tailor-made monolithic materials. Monolithic capillary columns will be specifically designed for the high throughput and/or multidimensional separations in microHPLC, as well as in capillary and thin layer electrochromatography. Using microHPLC, the separation of difficult protein mixtures will be explored using columns with carefully designed selectivities. Entirely new CEC separation modes enabled by the progress in grafted chemistries will be developed and used for the rapid and efficient separations of proteins and peptides. New approaches to thin layer chromatography and related methods making use of macroporous polymer sheets will be focused on the larger scale and 2-D separation of biomacromolecules and will include both pressure and electrodriven flow. This proposal also targets the development of an enhanced array of monolithic tools available for handling biological samples. Using the benefits of sequential photografting, monolithic devices will be prepared in the tip of electrospray emitters that will integrate sample collection, enzymatic digestion of proteins, and separation of peptides. Direct coupling of this device to mass spectrometer will simplify and accelerate proteomic research. Overall, our targets are to extend the applications of monolithic materials with both enhanced performance and unexpected capabilities in new territories and to demonstrate their vast potential in numerous areas.