Epigenetic profiling for detecting long lasting toxic effects on germline development in vitro

环境或药物对雄性或雌性生殖系的潜在影响仍然是毒理学中一个极其贫乏的领域，特别是在哺乳动物系统中。坊间研究提供了一些证据，表明药物(丙戊酸钠)或化学品(双酚-A)对生殖系产生影响，特别是对后代造成表观遗传变化，而不是遗传毒性影响。然而，其中许多研究的重复性和与人类的相关性受到了挑战，几乎没有关于这些变化如何发生、传递和延续到下一代的机械性信息(S)。该项目将使用表观遗传学图谱来解决这一问题，以检测对生殖系体外发育的长期毒性影响。

The potential impact of either environmental or pharmacological agents on the male or female germlines remains an incredibly poorly understood area of toxicology, particularly in mammalian systems. Studies have anecdotally provided some evidence of drugs (Sodium Valproate) or chemicals (Bisphenol-A) impacting on the germline, and particularly inducing epigenetic changes to subsequent generations, as opposed to genotoxic effects. However, the reproducibility and human relevance of many of these studies has been challenged, with little to no mechanistic information on how these changes may occur, be transmitted and persist into the next generation(s). This project will address this using epigenetic profiling for detecting long lasting toxic effects on germline development in vitro.