Forensic Detection of Salmonella Resistance and Subtype

描述（由申请人提供）：非伤寒沙门氏菌肠亚种肠（S. enterica）是造成全球食源性肠胃炎爆发的主要原因，并已被用于生物恐怖主义。此外，世界范围内耐抗生素肠链球菌的数量有所增加，包括整合子相关的多药耐药。由于肠炎沙门氏菌感染对军队和平民造成持续的生物恐怖威胁，这些发现值得关注。因此，快速发现疫情和耐药性，以及对用于生物恐怖主义的菌株进行法医分析，对公共安全至关重要。本项目旨在开发快速的分子方法来监测、检测和表征肠球菌暴发。该建议的核心是鉴定整合子介导的耐药决定因素和开发用于检测多重耐药肠链球菌的虚拟表型方法。该项目的具体目标是开发和验证1)标准化的高通量方法，用于快速检测和表征肠链球菌整合子介导的多耐药；2)快速检测肠链球菌血清型爆发的分子方法。

DESCRIPTION (provided by applicant): Non-typhoidal Salmonella enterica subspecies enterica (S. enterica) is responsible for a substantial proportion of global food-borne gastroenteritis outbreaks and has already been used for bioterrorism. In addition, there has been a worldwide increase in antibiotic-resistant S. enterica, including integron-associated multi-drug resistance. These findings are of concern due to the ongoing bioterrorist threat that S. enterica infection poses to military and civilian populations. Therefore, rapid detection of outbreaks and drug resistance, as well as forensic analysis of strains used for bioterrorism, are critical to public safety. This project proposes to develop rapid molecular methods for the surveillance, detection and characterization of S. enterica outbreaks. Central to this proposal is the identification of integron-mediated drug resistance determinants and the development of a virtual phenotype approach for the detection of multi-drug resistant S. enterica. The specific aims of the project are to develop and validate 1) standardized, high-throughput methods for rapid detection and characterization of integron-mediated MDR in S. enterica and 2) molecular methods for rapid outbreak detection among S. enterica serovars.