Core--Gene expression profiling

广泛的基因组测序工作和微阵列技术的进步已经融合在一起，为基因表达的高通量分析创造了革命性的新机会。在该计划的范围内，基因表达分析将提供关键信息， 阐明亨廷顿蛋白和DNA结合治疗剂改变基因的机制 表情基因表达谱核心将支持该计划的个别项目， 同时跟踪数千种mRNA在相应实验中的表达， 系统，从而鉴定给定病理或药理学的特定基因靶点。 操纵鉴定受特定效应影响的基因启动子将允许启动子 元件和转录因子负责的变化被阐明。基因 Expression Core将提供微阵列分析研究的设计、执行和分析方面的专业知识，并将使用独立的方法（如北方印迹法）进行验证性分析。该核心的主要研究者在基于微阵列的表达分析方面拥有丰富的经验，包括之前对多聚谷氨酰胺疾病小鼠模型的几项研究。

Extensive genome sequencing efforts and advances in microarray technology have converged to create revolutionary new opportunities for high-throughput analyses of gene expression. Within the context of the Program, gene expression analyses will provide information critical to elucidating the mechanisms by which huntingtin and DNA-binding therapeutic agents alter gene expression. The Gene Expression Profiling Core will support the Program's individual Projects by tracking the expression of thousands of mRNAs simultaneously in the respective experimental systems, thereby identifying the specific gene targets of a given pathological or pharmacologic manipulation. Identifying the gene promoters subject to a particular effect will allow the promoter elements and transcription factor(s) responsible for the change to be elucidated. The Gene Expression Core will provide expertise in the design, execution and analysis of microarray profiling studies and will carry out confirmatory analyses using independent methodologies, such as northern blotting. The Principal Investigator of the Core has extensive experience in microarray based expression analyses, including several previous studies of mouse models of polyglutamine disease.