Transgenic Chickens using Primordial Germ Cells

描述（由申请人提供）：Origen therapeutics的目标是开发能够产生完全人单克隆和多克隆抗体的鸡。为了实现这一目标，鸡Origen需要有能力插入和修改鸡的基因组，并通过种系传递这些基因组修改。Origen的平台围绕着一个基于细胞的系统，该系统允许整合大片段的DNA，并允许在基因组中精确删除。为此，Origen已经确定了一个有可能进入生殖系的细胞群。该应用侧重于用大的（150kb）构建体转染该细胞群，并敲除鸡基因组中的特定基因。然后将这些转染过的细胞群通过种系进行运输，以获得一群转基因鸡。

DESCRIPTION (provided by applicant): Origen Therapeutic's goal is to develop chickens that produce fully human monoclonal and polyclonal antibodies. To achieve this aim the chicken Origen needs the capability to insert and modify the chicken genome and to transmit these genomic modifications through the germline. Origen's platform centers around a cell based system that allows integration of large pieces of DNA and allows precise deletions in the genome. To this end Origen has identified a cell population that has the potential to enter the germline. This application focuses on transfecting this cell population with large (150 kb) constructs and to knock-out specific genes within the chicken genome. These transfected cell popupations will then be trafficked through the germline to obtain a flock of transgenic chickens.