IN VIVO DIFFERENTIATION AND MIGRATION PROPERTIES OF STEM CELLS

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。所列机构为中心，不一定是研究者所在机构。干细胞具有自我更新和分化成不同细胞类型的能力。成体间充质干细胞（MSC）在体外和体内分化为不同细胞类型的潜力为将这些细胞用作治疗剂提供了极好的机会。在这项研究中，骨髓和脂肪组织来源的MSC在体内的可塑性进行了比较。在时间进程研究中，将MSC注射到NIHIII免疫缺陷小鼠的侧脑室中。在植入后2、4和6周评价MSC的迁移和植入。使用与恒河猴细胞交叉反应的人核抗原（HNA）抗体筛选从移植脑制备的切片中的恒河猴细胞。我们的结果表明，虽然两种细胞类型在整个大脑的多个区域移植，但脂肪组织来源的MSC在大脑中持续更长的时间。移植后6周检测脂肪组织来源的MSC。我们目前正在研究移植的MSC分化到宿主脑中。由于使用成体干细胞没有伦理限制，这项研究的结果表明，脂肪组织和骨髓将被证明是多能干细胞的重要来源。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Stem cells have the capacity for self-renewal and differentiation into diverse cell types. The potential of adult mesenchymal stem cells (MSCs) to differentiate to diverse cell types in vitro and in vivo offers outstanding opportunities to use these cells as therapeutic agents. In this study bone marrow and adipose tissue derived MSCs were compared for the plasticity in vivo. In a time course study, MSCs were injected into the lateral ventricles of NIHIII immune deficient mice. The migration and engraftment of MSCs have been evaluated on 2, 4, and 6 weeks post engraftment. The sections made from the engrafted brain were screened for rhesus cells using Human Nuclear Antigen (HNA) antibody which cross reacted with rhesus cells. Our results indicated that while both cell types engrafted in multiple regions throughout the brain, the adipose tissue derived MSCs persisted in the brain for longer periods of time. The adipose tissue derived MSCs were detected up to six weeks post-transplantation. We are currently investigating the differentiation of engrafted MSCs into the host brain. Since there is no ethical limitation on using adult stem cells, the results of this study suggest that adipose tissue and bone marrow will prove to be important sources of pluripotent stem cells.